Materials and method for modifying a biochemical component in a plant

ABSTRACT

A method of modifying the amount of at least one biochemical component in a plant comprising expressing Qua-Quine Starch (QQS) in the plant, the wild-type of which does not express QQS; a transgenic plant, or part thereof, which comprises and expresses QQS as a transgene and in which the amount of at least one biochemical component is modified; a tissue culture of regenerable cells of the transgenic plant; a vector comprising a nucleotide sequence, which encodes the coding sequence of QQS, operably linked to a non-native promoter, which promotes expression of the nucleotide sequence in a plant, which is other than  Arabidopsis ; and a method of producing a food or industrial product from a plant.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a divisional of co-pending patent application Ser.No. 13/314,139, filed Dec. 7, 2011, which claims priority to U.S.provisional application No. 61/446,460, filed Feb. 24, 2011, thecontents of which are incorporated herein by reference in theirentireties.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

The invention disclosed herein was made with support from the Governmentof the United States of America under Grant Nos. MCB0209789 andMCB0951170 awarded by the National Science Foundation. The Governmenthas certain rights in the invention.

TECHNICAL FIELD

This disclosure relates to materials and a method for modifying theamount of at least one biochemical component in a plant, in particular acrop plant. The amount of carbohydrate, e.g., starch, in leaves and/orseeds and the amount(s) of protein and/or lipid, e.g., oil, in seeds ofplants can be modified in accordance with the present disclosure.Specifically, this disclosure relates to the expression of Qua-QuineStarch (QQS) in a plant (or a part thereof), in particular a crop plant,the WT of which does not otherwise express QQS.

BACKGROUND

Plant storage products include starch, lipid and protein. Differentplant species differ in the kinds and relative proportions of storageproducts. Grain seeds, for example, comprise mainly starch, whereas oilseeds comprise mainly oil, and other seeds, such as soybean seeds,comprise mainly protein.

The pathways that produce starch, lipid and protein are connected by keyintermediates. For example, hexoses, such as glucose, are precursors ofstarch synthesis and end products of starch degradation. Hexoses alsocan be converted to pyruvate, which can lead to (i) the production ofacetyl-CoA and malonyl-CoA for fatty acid de novo synthesis, fatty acidelongation, polyketide formation, and isoprenoid synthesis and (ii) thebiosynthesis of amino acids.

The ability to alter plant storage products, particularly for cropplants, has been a goal of plant breeders and those employingrecombinant DNA techniques. Mike Rankin, a Crops and Soils Agent for theUniversity of Wisconsin (UW), recently reported that Roger Borgers, asoybean specialist with UW has made a good case that low proteinsoybeans are costing the soybean industry as a whole due to discountedpricing for U.S. soybeans, which are comparatively low-protein, on theworld market (www.uwex.edu/ces/crops/SoyProtein.htm). According to thoseat the UW, soybeans from South America are valued more highly, andpriced accordingly. Reportedly, one bushel of U.S. soybeans yields 21pounds of protein and 11.4 pounds of oil, whereas Brazilian soybeansyield 24 pounds of protein and 13 pounds of oil. Rankin opines that moreemphasis needs to be put on developing varieties with higher proteincontent, particularly given that there is a wide variation in soybeanprotein content within U.S. geographic regions (0.6-2.0%, depending onthe year) and among varieties (32.3%-38.1% in Wisconsin). In thisregard, the western Corn Belt and Wisconsin do not fare as well as otherregions in the U.S., such as the southeast. Significant is that yield isnot reduced with higher protein and oil content. In this regard, Bakeret al. (Poultry Science 90: 390-395 (2011)) reported that soybean mealproduced from high protein or low oligosaccharide varieties of soybeanshave a greater nutritional value than soybean meal produced fromconventional varieties of soybean and, therefore, could be fed insmaller amounts to broiler chickens.

Recently, the qua-quine starch (QQS) gene (locus ID At3g30720; GenBankAccession Nos. EU805808.1 and NM_(—)113075.4) was found to have aneffect on plant biochemical components in Arabidopsis. The QQS geneencodes a protein that contains 59 amino acids, has no known function,has no sequence similarity to other proteins in Arabidopsis or otherorganisms, has no known catalytic motifs, and no known structuralmotifs. Analysis of the QQS promoter indicates that it has a CCA1binding site motif (AAAAATCT) at position −734, a TGA1 binding sitemotif (TGACGTGG; bZip transcription factor function) at position −504,an UPRMOTIFIAT motif (TGACGTGG; unfold protein response) at position−504, an ABRE-like binding site motif (GACGTGGC; ABA function) atposition −503, and an ACGTABREMPTIFA2OSEM motif (ACGTGGC; ABA function)at position −502. QQS RNA transcripts increase during pollen development(from uninucleate microspores to bicellular pollen to tricellular pollento mature pollen) in WT (WT) Arabidopsis, reaching peak levels in maturepollen. In wild type (WT) Arabidopsis, activity of the QQS promoter asdetermined using the β-glucuronidase (GUS) gene reporter system isevident at 2 days after imbibition (DAI) in hypocotyls and root tips. Asseedlings grow, QQS expression expands to the vasculature, mesophyllcells, hydathodes, and trichomes of leaf blades and petioles.Microscopic dissection indicates no expression is detected in shootmeristem; the dark GUS staining in the shoot tip is associated with theadjacent vasculature. GUS activity is higher in mature leaves comparedto young emerging leaves; it consistently appears somewhat unevenlydistributed, and is predominantly located in the vasculature; thispattern is maintained throughout development. QQS expression is low inflower buds; however, by flower opening QQS expression is evident inpedicels, sepals, filaments, mature pollen, stigma papillae and styles,but not in petals. During silique development, QQS expression rises inthe stigma papillae and style, and becomes apparent throughout thematernal tissues of the silique wall and receptacle. QQS is expressed inroots throughout development. Expression is highest in the root tip,specifically the root cap, columella cells and peripheral cap, and to alesser extent in the root meristem region, but not in the epidermis. QQSis expressed at the site of lateral root initiation, and in the root tipand vasculature during its emergence; as the lateral root matures,expression remains detectable throughout the root cortex vasculature.GUS activity driven by the QQS promoter was higher in the Atss3 (starchsynthase 3) single mutant than in WT under virtually all conditions.Expression was detectable throughout the entire seedling at 2 DAI, aswell as later in development, in particular in leaves, flowers androots. Although the general pattern of expression is similar in theAtss3 mutant and WT, QQS is expressed ectopically in petals in the Atss3mutant. QQS RNA accumulates neither in the nucleus nor in the plastids.Expression of QQS promoter-GUS in the Atss2/Atss3 double-mutantbackground was more nuanced, but was in general similar to or somewhatlower than that in WT throughout leaf development. QQS transcriptsincreased seven-fold during the diurnal cycle in the Atss3 mutantcompared to WT Arabidopsis; QQS protein levels also increased in theAtss3 mutant compared to WT Arabidopsis. Analysis of QQS RNAi(interfering RNA) mutants showed that starch content increased 20-30% atthe end of the light cycle (about the same increase as observed in Atss3mutants) due to increased starch biosynthesis and not decreased starchdegradation; there was no difference in starch content at the end of thedark cycle. Starch content decreased to WT level within four hours ofthe dark cycle. All of the above examples are described in Li et al.,Plant Journal 58: 485-498 (2009).

In view of the foregoing, it is an object of the present disclosure toprovide materials and a method of modifying the amount of at least onebiochemical component in a plant. This and other objects and advantages,as well as inventive features, will become apparent from the detaileddescription provided herein.

SUMMARY

A method of modifying the amount of at least one biochemical componentin a plant is provided. The method comprises expressing Qua-Quine Starch(QQS) in a plant, the WT of which does not express QQS, in an amountthat modifies the amount of at least one biochemical component in theplant. The biochemical component can be protein, lipid (e.g., oil),polyketide, isoprenoid, and/or carbohydrate (e.g., starch). When thebiochemical component is protein, the amount of protein in the seeds ofthe plant expressing QQS can be increased compared to the amount ofprotein in the seeds of the WT plant. When the biochemical component isoil, the amount of oil in the seeds of the plant expressing QQS can bedecreased compared to the amount of oil in the seeds of the WT plant.When the biochemical component is starch, the amount of starch in theleaves of the plant expressing QQS can be decreased compared to theamount of starch in the leaves of the WT plant.

When the plant is soybean and the biochemical component is protein, theamount of protein in the seeds of the soybean can be increased by atleast about 30% as compared to the amount of protein in the seeds of WTsoybean. The amount of protein in the seeds of the soybean can beincreased by at least about 45%, such as by at least about 60%.

In view of the above, also provided is a transgenic plant, or partthereof, which comprises and expresses QQS as a transgene. In thetransgenic plant, or part thereof, at least one biochemical component ispresent in an amount that differs from the amount present in acorresponding WT plant, or part thereof, which does not express QQS. Thetransgenic plant, or part thereof, can be soybean.

Also in view of the above, a tissue culture of regenerable cells of atransgenic plant (or part thereof), which comprises and expresses QQS asa transgene, is provided. At least one biochemical component is presentin the transgenic plant in an amount that differs from the amountpresent in a corresponding WT plant, or part thereof, which does notexpress QQS.

A vector is further provided. The vector comprises a nucleotidesequence, which encodes the coding sequence of QQS, operably linked to anon-native promoter, which promotes expression of the amino acidsequence of the nucleotide sequence in a plant, wherein the plant isother than Arabidopsis. The nucleotide sequence preferably encodes theamino acid sequence of SEQ ID NO: 2. An example of such a nucleotidesequence is SEQ ID NO: 1. The promoter can be any suitable promoter,such as a constitutive promoter, e.g., a Cauliflower mosaic virus 35Spromoter, an inducible promoter, a developmentally specific promoter,such as a seed-specific promoter, or a synthetic promoter, e.g., ahybrid promoter.

A method of producing a food or industrial product from a plant is stillfurther provided. The method comprises preparing the food or industrialproduct from a cultivated transgenic plant or a cultivated plantregenerated from a tissue culture of regenerable cells of a transgenicplant (or part thereof). The transgenic plant comprises and expressesQQS as a transgene. At least one biochemical component is present in thetransgenic plant in an amount that differs from the amount present in acorresponding WT plant, or part thereof, which does not express QQS.While the transgenic plant, or part thereof, can be any plant in whichthe modification of the amount of a biochemical component is desired (asindicated above), a preferred plant is soybean.

BRIEF DESCRIPTION OF FIGURES

FIG. 1 a is the nucleotide sequence of the Arabidopsis thalianaQua-Quine Starch (QQS) cDNA [SEQ ID NO: 1].

FIG. 1 b is the amino acid sequence of the Arabidopsis thaliana QQSprotein [SEQ ID NO: 2].

FIG. 2 is a bar graph of starch content (mg/g fresh weight), oil (% dryweight), and protein (% dry weight) in transgenic soybean lines vs. WT(Williams 82) soybean.

FIG. 3 is a drawing of the construction of a vector (QQS-OE At3g30720)for expression of QQS in plants other than Arabidopsis, wherein Hpa I,Stu I and Apa I are restriction endonuclease sites, Sm/SpR is a generesistant to spectinomycin, LB is the left transfer-DNA border, Bar is agene that confers resistance to the herbicide bialaphos, p35S is theCauliflower mosaic virus (CaMV) 35S promoter, attB1 and attB2 areGateway DNA recombination sites B1 and B2, T35S is the terminator of theCaMV 35S promoter, RB is the right transfer-DNA border, and QQS CDS isthe coding sequence for QQS (180 nts; nucleotides 301-480 of SEQ ID NO:1).

DETAILED DESCRIPTION

The present disclosure is predicated, at least in part, on thesurprising and unexpected discovery that the qua-quine starch (QQS) gene(locus tag At3g30720; GenBank Accession Nos. EU805808.1 andNM_(—)113075.4), which encodes a protein that contains 59 amino acids,has an effect on biochemical components in Arabidopsis, has no sequencesimilarity to other proteins in Arabidopsis or other organisms, has noknown catalytic motifs, and no known structural motifs, also has aneffect on biochemical components in other plants. The ability to modifythe amount of at least one biochemical component in a plant byexpressing QQS in the plant enables the generation of plant lines withmore desirable biochemical component compositions without conductingextensive breeding studies.

In view of the above, a method of modifying the amount of at least onebiochemical component in a plant is provided. The method comprisesexpressing QQS in the plant, the WT of which does not express QQS, in anamount (i.e., an effective amount, e.g., a biochemicalcomponent-modifying amount), or at a level (i.e., an effective level,e.g., a biochemical component-modifying level), that modifies the amountof at least one biochemical component in the plant. When QQS isexpressed in the plant (the wild-type of which does not express QQS) andthe amount of at least one biochemical component in the plant ismodified, QQS is being expressed in the plant in an amount, or at alevel, that modifies the amount of at least one biochemical component inthe plant. The method can be used to produce a food product (or acomponent or an ingredient thereof) for human consumption, feed (or acomponent or an ingredient thereof) for non-human animal consumption, oran industrial product (i.e., any and all non-food products, orcomponents or ingredients thereof), such as in accordance with methodsdescribed herein. In this regard, the “biochemical component,” asdescribed herein below, e.g., a biochemical component, which isincreased in amount, or a plant (or part thereof), in which abiochemical component is increased/decreased in amount, can be the foodproduct (or a component or an ingredient thereof), the feed (or acomponent or an ingredient thereof), or the industrial product (or acomponent or an ingredient thereof).

By “biochemical component” is meant any fraction of the composition of aplant (e.g., carbon fraction, nitrogen fraction, phosphorus fraction, orion fraction), any class of compounds/ions of which a fraction iscomprised (e.g., polysaccharides, sugars, organic acids, phenolics,tannins, hemicelluloses, lipids, terpenoids, cellulose and lignin of thecarbon fraction; protein, amino acid, alkaloid, nitrate and nucleic acidof the nitrogen fraction; phosphate, polyphosphate, phospholipid,nucleic acid, and sugar phosphate of the phosphorus fraction; andpotassium, calcium, magnesium, salt, and heavy metal of the ionfraction), and any compound of which a class of compounds is comprisedthat is built-up in a plant and can be mobilized to support biosynthesisfor growth or other plant functions (see, e.g., Chapin, I I I et al.,Ann. Rev. of Ecology and Systematics 21: 423-447 (1990) and referencescited therein, all of which are hereby incorporated by reference intheir entireties). Examples of plant lipid classes include neutrallipids, such as triacylglycerol, diacylglycerol, and monoacylglycerol,and polar lipids, such as monogalactosyldiacylglycerol,digalactosyldiacylglycerol, phosphatidylglycerol, phosphatidylcholine,phosphatidylethanolamine, phosphatidylinositol, phsophatidylserine, andsulfoquinovosyldiacylglycerol. Common plant fatty acids include palmiticacid, palmitoleic acid, palmitolenic acid, stearic acid, oleic acid,linoleic acid, linolenic acid, arachidic acid, eicosenoic acid,eicosadienoic acid, and erucic acid. A “biochemical component” isintended to encompass a compound that can be formed by one or more typesof storage processes (e.g., starch and amino acids) and a compound thatcan serve a storage role and a non-storage role (e.g., ribulosebis-phosphate carboxylase (RUBISCO) and tannins). The biochemicalcomponent can be protein, lipid (e.g., oil), polyketide, isoprenoid,and/or carbohydrate (e.g., starch).

By “modifying” is meant increasing or decreasing the amount of abiochemical component. In this regard, the expression of QQS in theplant can modify one or more biochemical components by increasing theamount of one or more biochemical components, decreasing the amount ofone or more biochemical components, or simultaneously increasing theamount of one of more biochemical components and decreasing the amountof one or more other biochemical components. Thus, the biochemicalcomponent can be protein, and the amount of protein in the seeds, forexample, of the plant expressing QQS can be increased compared to theamount of protein in the seeds of the WT plant. Additionally oralternatively, the biochemical component can be lipid (e.g., oil), andthe amount of lipid (e.g., oil) in the seeds, for example, of the plantexpressing QQS can be decreased compared to the amount of lipid (e.g.,oil) in the seeds of the WT plant. Also, additionally or alternatively,the biochemical component can be carbohydrate (e.g., starch), and theamount of carbohydrate (e.g., starch) in the leaves, seeds, or leavesand seeds, for example, of the plant expressing QQS can be decreasedcompared to the amount of carbohydrate (e.g., starch) in the leaves,seeds, or leaves and seeds, respectively, of the WT plant. The amount ofat least one biochemical component can be modified, such as byincreasing or decreasing, by at least about 5%, 10%, 15%, 20%, 25%, 30%,35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or more.For example, as demonstrated herein, when the plant is soybean and thebiochemical component is protein, the amount of protein in the seeds ofthe soybean can be increased by at least about 30%, such as 32.63%(based on dry weight), at least about 45%, such as 47.08% (based on dryweight), at least about 60%, such as 60.63% (based on dry weight), ormore.

When the amount of at least one biochemical component is modified byincreasing the amount of the biochemical component, it can be preferableand even desirable to supplement the growing conditions of the plant,such as soil or other media in which the plant is grown, with acomposition that provides one or more nutrients essential to the growthof the plant and/or the increased production of at least one biochemicalcomponent in the plant. The composition can be inorganic (e.g.,mineral), organic (e.g., derived from a plant and/or animal), or acombination thereof. The composition can be of natural origin, syntheticorigin, or a combination thereof. An example of such a composition is afertilizer (e.g., Miracle Gro Excel 15-5-15). A fertilizer typicallyprovides, in varying proportions, macronutrients, such as nitrogen,phosphorus, potassium, calcium, magnesium, and sulfur. A fertilizer alsotypically provides, in varying proportions, micronutrients, such asboron, chlorine, copper, iron, manganese, molybdenum, and zinc. Othernutrients, such as carbon, hydrogen, and oxygen, are typically suppliedby water and carbon dioxide. If desired, the composition (e.g.,fertilizer) can be a controlled-release composition. In this regard, thecomponents of the controlled-release composition can be released at thesame rate or at different rates and/or in the same amounts or indifferent amounts. An excess amount of a given biochemical component canbe released from a plant, which expresses a biochemical-componentmodifying amount/level of QQS, into its environment as part of theplant's normal growth/development or life cycle.

The plant can be any plant in which the modification of the amount of abiochemical component is desired. For example, the plant can be amonocot, a dicot, or a fungus. Examples of plants include agriculturallyimportant plants, such as cereal crops, industrial plants, legumes,fruits, vegetables, root plants, turf grasses, woody plants, tropicalplants, nuts, ornamental plants, medicinal plants, and fungi, amongothers, and, more specifically, corn, soybean, wheat, barley, oat, flax,kiwicha, bulgur, quinoa, millet, sorghum, sugarcane, potato, sweetpotato, cotton, rice (e.g., indica and japonica), rye, canola, oilseedrape, sunflower, tobacco, beans, alfalfa, Bermudagrass, perennialryegrass, switchgrass, tall fescue, turf grasses, American elm, Americanchestnut, cork oak tree, eucalyptus, pine, poplar, rubber tree, banana,blackberry, blueberry, strawberry, raspberry, cantaloupe, melon,cucumber, eggplant, tomato, lettuce, radish, mushroom, carrot, cassava,onion, lentil, pea, chickpea, pigeonpea, cowpea, red clover, bean, limabean, kidney bean, broad bean, velvet bean, tepary bean, pepper,broccoli, spinach, squash, pumpkin, mustard, mustard greens, Indianmustard, apple, pear, peach, cherry, plum, grape, cabbage, cauliflower,brussel sprouts, citrus, orange, lemon, lime, grapefruit, tangerine,clementine, pomegranate, kiwi, starfruit, anise, papaya, pineapple,coffee, groundnut, palm kernel, walnut, peanut, almond, pecans,chestnuts, macadamia nuts, hazelnuts, sunflower, endive, leek, beet,turnip, clover, red clover, barrel clover, carnation, chrysanthemum,orchids, petunia, rose, ginseng, hemp, opium poppy, African locust bean,African oil bean, tarwi, tamarind, Mung bean, Sesban bean, Lablab bean,Jack-bean, lupin, and milkweed.

QQS can be expressed in the plant using any suitable method as is knownin the art. For example, QQS can be introduced into the plant as atransgene using electroporation, microprojectile bombardment,Agrobacterium-mediated transformation (as exemplified herein in Example1), and direct contact of protoplasts. Transformation/transfection (aswell as other techniques used to introduce DNA into a plant or fungus)and regeneration of monocots and dicots is a matter of routine. Theparticular method employed will depend, in part, on the type of plant orfungus to be transformed/transfected. For example, numerous protocolsare described in Agrobacterium Protocols, 2^(nd) ed., Vols. 1 and 2,Methods in Molecular Biology, which was edited by Kan Wang and publishedby Humana Press, Totowa, N.J., and which is specifically incorporatedherein by reference in its entirety. Such protocols include the floraldip transformation method and methods of transforming leaf explants,cotyledon explants, and root explants, as well as specific protocols fortransformation of barrel clover, tobacco, barley, corn, rice (indica andjaponica), rye, sorghum, wheat, canola, cotton, Indian mustard,sunflower, alfalfa, chickpea, clover, pea, peanut, pigeonpea, redclover, soybean, tepary bean, taro, cabbage, cucumber, eggplant,lettuce, tomato, carrot, cassava, potato, sweet potato, yam,Bermudagrass, perennial ryegrass, switchgrass, tall fescue, turfgrasses, American elm, cork oak, eucalyptus tree, pine, poplar, rubbertree, banana, citrus, coffee, papaya, pineapple, sugarcane, Americanchestnut, apple, blueberry, grapevine, strawberry, walnut, carnation,chrysanthemum, orchids, petunia, rose, ginseng, hemp, opium poppy, andmushroom. Other methods of Agrobacterium-mediated transformation ofcereals are described by Shrawat et al., Plant Biotech. J. 4(6): 575-603(November 2006), which is specifically incorporated herein by referencein its entirety. Other methods of transformation of legumes aredescribed by Somers et al., Plant Physiol. 131(3): 892-899 (March 2003),which is specifically incorporated herein by reference in its entirety.Methods useful for the transformation of rice are described by Giri etal., Biotech. Adv. 18(8): 653-683 (December 2000), and Hiei et al.,Plant Mol. Biol. 35(1-2): 205-218 (September 1997), both of which arespecifically incorporated herein by reference in their entireties. Vasilet al., Methods Molec. Biol. 111: 349-358 (1999), and Jones et al.,Plant Methods 1(1): 5 (Sep. 5, 2005), both of which are specificallyincorporated herein by reference in their entireties, describe methodsuseful for the transformation of wheat. The use of direct DNA uptake inbarley has been described by Lazzeri, Methods Molec. Biol. 49: 95-106(1996), which is specifically incorporated herein by reference in itsentirety. The use of temporary immersion in a bioreactor system totransform strawberries is described by Hanhineva et al., BMC Biotech. 7:11 (2007), which is specifically incorporated herein by reference in itsentirety. The introduction of transgenes into plastids, such aschloroplasts, specifically chloroplasts in tobacco, has been describedby Daniell et al., Trends Biotech. 23(5): 238-245 (May 2005), which isspecifically incorporated herein by reference in its entirety. In thisregard, Lutz et al., Plant Physiol. 145(4): 1201-1210 (2007)(specifically incorporated herein by reference in its entirety),provides guidance in the selection of vectors for transformation of theplastid genome in higher plants. Somatic embryogenesis ofspecies-specific chloroplast vectors also has application in plants,such as soybean, carrot, and cotton, for example. Other methods usefulfor the transformation of beets have been described by Golovko et al.,Tsitol. Genet. 39(3): 30-36 (May-June 2005), which is specificallyincorporated herein by reference in its entirety.

A nucleotide sequence, which encodes the coding domain sequence (CDS) ofQQS, can be incorporated into a vector or a cassette (collectivelyreferred to herein as vectors) for expression in a plant. Numerousexpression vectors suitable for stable transformation of plant cells orfor the establishment of transgenic plants have been described (see,e.g., Weissbach et al., Methods for Plant Molecular Biology, AcademicPress, New York, N.Y. (1989); and Gelvin et al., Plant Molecular BiologyManual, Kluwer Academic Publishers, Norwell, Mass. (1990)). The Tiplasmid from Agrobacterium tumefaciens or a binary Agrobacterium vector(Bevan, Nucl. Acids Res. 12: 8711-8721 (1984)) can be used to transformmonocots and dicots. Non-Ti vectors, such as viral vectors, can be usedto transfer DNA into plant cells, tissues, embryos, and plants. Non-Tivectors can be introduced through the use of liposome-mediatedtransformation, polyethylene glycol (PEG)-mediated transformation, viraltransfection, micro-injection, vacuum infiltration, electroporation ofplant protoplasts, microprojectile bombardment, silicon carbide wiskers,and the like. See, e.g., Ammirato et al., Handbook of Plant CellCulture—Crop Species, MacMillan Pub. Co. (1984); Shimamoto et al.,Nature 338: 274-276 (1989); Fromm et al., Bio/Technology 8: 833-839(1990); and Vasil et al., Biol. Technology 8: 429-434 (1990).

In addition to a coding sequence, a plant transformation/transfectionvector comprises one or more 5′ and 3′ transcriptional regulatorysequences. Transcriptional regulatory sequences can include a promoter,a transcription initiation site, a transcription termination site, apolyadenylation signal, and a 3′ terminator region (e.g., PI-IIterminator region of potato, octopine synthase 3′ terminator region, ornopaline synthase 3′ terminator region). If a conventional, nuclearprocessed intron is present, one or more RNA processing signals, such asintron splice sites, also can be included. Any suitable promoter can beused. In this regard, the QQS promoter can be used. Alternatively, anon-QQS promoter can be used. The promoter can be constitutive,synthetic (e.g., hybrid), inducible, developmentally regulated,environmentally regulated, hormonally regulated, chemically regulated,cell-specific, or tissue-specific (e.g., seed-specific), for example.Constitutive promoters include the cauliflower mosaic virus (CaMV) 35Spromoter, the nopaline synthase promoter, and the octopine synthasepromoter. Environmentally regulated, inducible promoters includepromoters that are induced by light, for example. The napin promoter,the phaseolin promoter, and the DC3 promoter are examples ofseed-specific promoters, whereas the dru1 promoter, the 2A11 promoter,and the tomato polygalacturonase promoter are examples of fruit-specificpromoters, and PTA29, PTA26, and PTA13 are examples of pollen-specificpromoters. The pBAN promoter is a seed coat promoter in Arabidopsis,whereas p26, p63, and p63tr are early seed promoters from Arabidopsis(see, e.g., U.S. Pat. App. Pub. No. 2009/0031450). Examples ofroot-specific promoters are described in U.S. Pat. Nos. 5,618,988;5,837,848; and 5,905,186. Other promoters are induced by auxin,cytokinin, gibberellin, methyl jasmonate, salicylic acid, heat, light,and the like.

In view of the above, a vector comprising a nucleotide sequence, whichencodes the coding sequence of QQS, operably linked to a non-nativepromoter, which promotes expression of the nucleotide sequence in aplant, wherein the plant is other than Arabidopsis, is also provided.The nucleotide sequence preferably encodes the amino acid sequence ofSEQ ID NO: 2. An example of such a nucleotide sequence is SEQ ID NO: 1,although one of ordinary skill in the art will appreciate that, due tothe degeneracy of the genetic code, numerous other nucleotide sequencescan encode the amino acid sequence of SEQ ID NO: 2. In this regard, oneof ordinary skill in the art will also appreciate that one or moremutations can be introduced into the nucleotide sequence, therebyleading to changes in the amino acid sequence of QQS. If a mutation isintroduced into the amino acid sequence of QQS, preferably the mutationdoes not alter the function of QQS or it improves the function of QQS insome way. By “non-native” is meant that the promoter is other than thatwhich promotes the expression of QQS in WT Arabidopsis. “Non-native” isintended to encompass the use of other Arabidopsis promoters, i.e.,other non-QQS promoters. As indicated above, the promoter can be anysuitable promoter, such as a constitutive promoter, e.g., thecauliflower mosaic virus 35S promoter, a synthetic promoter (e.g.,hybrid), an inducible promoter, a developmentally specific promoter,e.g., a seed-specific promoter, and a synthetic promoter, e.g., a hybridpromoter. Promoters that are highly active in soybean, e.g., aconstitutive polyubiquitin promoter and an early embryo-specific heatshock protein 90-like promoter, are described in U.S. Pat. App. Pub. No.2010/0186119. The method of modifying the amount of at least onebiochemical component in a plant described herein is not limited to theuse of a vector comprising a nucleotide sequence encoding the codingsequence of QQS operably linked to a non-native promoter; a vectorcomprising a nucleotide sequence encoding the coding sequence of QQSoperably linked to the QQS promoter also can be used.

Following transformation/transfection (or other such methods ofintroducing DNA into a plant), plants can be selected using a dominantselectable marker (e.g., antibiotic or herbicide resistance)incorporated into the vector/cassette. After transformed/transfectedplants are selected, they are grown to maturity. Plants showing amodified amount of at least one biochemical component are identified.Modulation can be confirmed through analysis of mRNA expression usingNorthern blots, RT-PCR, micro-arrays, or next generation sequencing, orthrough analysis of protein expression/accumulation using immunoblots,Western blots, or gel shift assays.

In view of the above, a transgenic plant, or part thereof, is alsoprovided. The transgenic plant, or part thereof, comprises and expressesQQS as a transgene and at least one biochemical component is present inan amount that differs from the amount present in a corresponding WTplant, or part thereof, which does not express QQS. By “part thereof” ismean any part of a plant such as, but not limited to, root, stem, leaf,flower, stamen, pollen, pistil, seed, and the like. The transgenic plantcan be any plant as indicated above, such as, but not limited to,soybean.

Also provided is a tissue culture of regenerable cells of the transgenicplant. By “tissue culture” is meant a composition comprising isolatedcells, which can be the same or different, or a collection of such cellsorganized into one or more parts of a plant. Exemplary types of tissuecultures include an explant, a callus, an embryo, and a plantlet. By“regenerable” is meant that the cells can regenerate a plant like thetransgenic plant from which the tissue culture was derived. Plant tissueculture techniques are known in the art (see, for example, Smith, PlantTissue Culture: Techniques and Experiments, 2nd. ed., Academic Press(2000); and Plant Tissue Culture, Development, and Biotechnology,Trigiano and Gray, eds., CRC Press (2010), both of which areincorporated by reference in their entireties) and exemplified herein(see Example 2).

A method of producing a food or industrial product is also provided.“Food” includes food (or a component or an ingredient thereof) for humanconsumption as well as feed (or a component or an ingredient thereof)for non-human animal consumption. “Industrial” includes any and allnon-food products (or components or ingredients thereof). The method cancomprise preparing the food product (or a component or an ingredientthereof) or the industrial product (or a component or an ingredientthereof) from a cultivated transgenic plant as described above, e.g.,soybean, or a cultivated plant regenerated from a tissue culture asdescribed above. Thus, the present disclosure provides an improvedmethod of producing a food product or an industrial product, wherein theimprovement comprises preparing the food product or the industrialproduct from a cultivated transgenic plant or a cultivated plantregenerated from a tissue culture as described above.

Soybeans, for example, can be used in their entireties but are commonlyprocessed into two primary products, i.e., soybean protein (meal) andcrude soybean oil. Both of these products are commonly further refinedfor particular uses. The crude soybean oil can be broken down intoglycerol, fatty acids, and sterols. The soybean protein can be dividedinto soy flour concentrates and isolates. Examples of “food” productsmade from soybean include, but are not limited to, coffee creamers,margarine, mayonnaise, salad dressings, shortenings, bakery products,chocolate coatings, cereal, beer, aquaculture feed, bee feed, calf feedreplacers, fish feed, livestock feed, poultry feed, and pet feed.Examples of “industrial” products include, but are not limited to,binders, wood composites, anti-static agents, caulking compounds,solvents, disinfectants, fungicides, inks, paints, protective coatings,wallboard, anti-foam agents, and rubber.

If desired, the plants described herein can be used in plant breedingmethods. For example, plant breeding methods can be used to introduceone or more other traits, e.g., higher yield, into the plants describedherein. See, for example, the methods described in U.S. Pat. App. Pub.No. 2004/0060082, which published Mar. 25, 2004, and is herebyincorporated by reference in its entirety.

The methods described herein can be used to assess other proteins withobscure features (POF) in plants. Desirably, the POF shares certaincharacteristics with QQS, such as no sequence similarity to otherproteins within the same plant, or no sequence similarity to otherproteins in other plant species, such as related plant species withinthe same family, no known catalytic motifs, and no known structuralmotifs. Initially, the POF can be identified by comparing its primaryamino acid sequence to other amino acid sequences in databases, such asPFAM, TIGRFAM, SMART, and Superfamily, using a statistical model of aconsensus sequence for a group of homologous and/or orthologouspolypeptides, such as a Hidden Markov Model (HMM) (see, e.g., Durbin etal., Biological Sequence Analysis Probabilistic Models of Proteins andNucleic Acids, Cambridge University Press, Cambridge, UK (1988)), andfinding no matches (Gollery et al., Genome Biol. 7: R57 (2006)).Examples of POFs identified in Arabidopsis by this method include: LocusID AT1G01110, AT1G01130, AT1G01240, AT1G01400, AT1G01440, AT1G01500,AT1G01550, AT1G01725, AT1G01730, AT1G01810, AT1G01840, AT1G01990,AT1G02070, AT1G02110, AT1G02290, AT1G02320, AT1G02350, AT1G02380,AT1G02405, AT1G02450, AT1G02490, AT1G02540, AT1G02570, AT1G02700,AT1G02710, AT1G02870, AT1G02960, AT1G02965, AT1G02990, AT1G03055,AT1G03080, AT1G03090, AT1G03106, AT1G03170, AT1G03180, AT1G03200,AT1G03240, AT1G03260, AT1G03290, AT1G03320, AT1G03340, AT1G03420,AT1G03600, AT1G03660, AT1G03730, AT1G03780, AT1G03820, AT1G03910,AT1G04000, AT1G04030, AT1G04070, AT1G04200, AT1G04230, AT1G04330,AT1G04650, AT1G04660, AT1G04670, AT1G04750, AT1G04800, AT1G04930,AT1G04985, AT1G05040, AT1G05060, AT1G05065, AT1G05085, AT1G05090,AT1G05205, AT1G05210, AT1G05220, AT1G05320, AT1G05330, AT1G05340,AT1G05360, AT1G05385, AT1G05420, AT1G05430, AT1G05440, AT1G05450,AT1G05490, AT1G05575, AT1G05720, AT1G05780, AT1G05860, AT1G05894,AT1G06010, AT1G06135, AT1G06225, AT1G06240, AT1G06320, AT1G06420,AT1G06475, AT1G06500, AT1G06510, AT1G06540, AT1G06660, AT1G06770,AT1G06920, AT1G06923, AT1G06930, AT1G06950, AT1G06980, AT1G07020,AT1G07040, AT1G07060, AT1G07090, AT1G07135, AT1G07190, AT1G07290,AT1G07300, AT1G07330, AT1G07473, AT1G07476, AT1G07500, AT1G07680,AT1G07690, AT1G07795, AT1G07860, AT1G07910, AT1G07970, AT1G07985,AT1G07990, AT1G08020, AT1G08030, AT1G08035, AT1G08060, AT1G08180,AT1G08300, AT1G08380, AT1G08480, AT1G08520, AT1G08530, AT1G08580,AT1G09415, AT1G09470, AT1G09483, AT1G09645, AT1G09812, AT1G09950,AT1G09995, AT1G10100, AT1G10140, AT1G10155, AT1G10170, AT1G10180,AT1G10220, AT1G10250, AT1G10380, AT1G10385, AT1G10417, AT1G10420,AT1G10522, AT1G10530, AT1G10620, AT1G10650, AT1G10660, AT1G10690,AT1G10710, AT1G10790, AT1G10800, AT1G10840, AT1G10990, AT1G11070,AT1G11120, AT1G11240, AT1G11320, AT1G11400, AT1G11430, AT1G11440,AT1G11470, AT1G11480, AT1G11520, AT1G11655, AT1G11690, AT1G11850,AT1G11905, AT1G11915, AT1G12020, AT1G12040, AT1G12064, AT1G12080,AT1G12330, AT1G12380, AT1G12450, AT1G12530, AT1G12660, AT1G12670,AT1G12805, AT1G12810, AT1G12830, AT1G12845, AT1G13030, AT1G13220,AT1G13360, AT1G13390, AT1G13605, AT1G13607, AT1G13608, AT1G13609,AT1G13620, AT1G13650, AT1G13670, AT1G13755, AT1G13930, AT1G13990,AT1G14180, AT1G14280, AT1G14345, AT1G14450, AT1G14620, AT1G14630,AT1G14710, AT1G14755, AT1G14990, AT1G15010, AT1G15215, AT1G15230,AT1G15260, AT1G15270, AT1G15280, AT1G15320, AT1G15350, AT1G15385,AT1G15400, AT1G15415, AT1G15590, AT1G15600, AT1G15610, AT1G15620,AT1G15630, AT1G15640, AT1G15757, AT1G15770, AT1G15800, AT1G15825,AT1G15830, AT1G15840, AT1G15900, AT1G15940, AT1G16000, AT1G16020,AT1G16025, AT1G16080, AT1G16170, AT1G16500, AT1G16515, AT1G16630,AT1G16690, AT1G16730, AT1G16770, AT1G16790, AT1G16810, AT1G16840,AT1G16850, AT1G16860, AT1G16910, AT1G16950, AT1G16960, AT1G17030,AT1G17080, AT1G17090, AT1G17140, AT1G17270, AT1G17285, AT1G17300,AT1G17345, AT1G17350, AT1G17360, AT1G17400, AT1G17490, AT1G17510,AT1G17665, AT1G17780, AT1G17870, AT1G17900, AT1G17940, AT1G18000,AT1G18010, AT1G18060, AT1G18190, AT1G18220, AT1G18290, AT1G18380,AT1G18470, AT1G18510, AT1G18620, AT1G18730, AT1G18750, AT1G18810,AT1G18840, AT1G18850, AT1G18950, AT1G19010, AT1G19020, AT1G19030,AT1G19240, AT1G19330, AT1G19360, AT1G19394, AT1G19397, AT1G19400,AT1G19500, AT1G19520, AT1G19530, AT1G19620, AT1G19960, AT1G19980,AT1G19990, AT1G20070, AT1G20100, AT1G20180, AT1G20280, AT1G20290,AT1G20310, AT1G20430, AT1G20460, AT1G20530, AT1G20640, AT1G20690,AT1G20770, AT1G20830, AT1G20890, AT1G20970, AT1G21010, AT1G21020,AT1G21050, AT1G21170, AT1G21323, AT1G21330, AT1G21360, AT1G21370,AT1G21390, AT1G21395, AT1G21475, AT1G21500, AT1G21510, AT1G21520,AT1G21560, AT1G21580, AT1G21600, AT1G21610, AT1G21695, AT1G21740,AT1G21770, AT1G21830, AT1G21840, AT1G21940, AT1G21950, AT1G22010,AT1G22030, AT1G22060, AT1G22110, AT1G22120, AT1G22140, AT1G22230,AT1G22260, AT1G22275, AT1G22335, AT1G22420, AT1G22470, AT1G22590,AT1G22600, AT1G22680, AT1G22770, AT1G22790, AT1G22850, AT1G22885,AT1G22890, AT1G22970, AT1G22980, AT1G23040, AT1G23050, AT1G23060,AT1G23110, AT1G23150, AT1G23170, AT1G23230, AT1G23270, AT1G23510,AT1G23530, AT1G23540, AT1G23650, AT1G23830, AT1G23840, AT1G23850,AT1G24050, AT1G24060, AT1G24145, AT1G24160, AT1G24270, AT1G24310,AT1G24370, AT1G24380, AT1G24390, AT1G24460, AT1G24560, AT1G24575,AT1G24600, AT1G24706, AT1G24822, AT1G24851, AT1G24938, AT1G24996,AT1G25025, AT1G25097, AT1G25112, AT1G25170, AT1G25180, AT1G25275,AT1G25400, AT1G25425, AT1G25540, AT1G26110, AT1G26140, AT1G26180,AT1G26210, AT1G26290, AT1G26350, AT1G26600, AT1G26650, AT1G26665,AT1G26710, AT1G26720, AT1G26750, AT1G27020, AT1G27030, AT1G27090,AT1G27300, AT1G27385, AT1G27435, AT1G27510, AT1G27550, AT1G27610,AT1G27640, AT1G27670, AT1G27690, AT1G27695, AT1G27710, AT1G27790,AT1G27850, AT1G27990, AT1G28060, AT1G28080, AT1G28100, AT1G28135,AT1G28140, AT1G28150, AT1G28190, AT1G28240, AT1G28250, AT1G28260,AT1G28327, AT1G28375, AT1G28395, AT1G28400, AT1G28530, AT1G28540,AT1G28560, AT1G28630, AT1G28760, AT1G29010, AT1G29170, AT1G29179,AT1G29195, AT1G29270, AT1G29290, AT1G29300, AT1G29355, AT1G29480,AT1G29530, AT1G29540, AT1G29560, AT1G29580, AT1G29610, AT1G29620,AT1G29820, AT1G29830, AT1G29970, AT1G29980, AT1G30050, AT1G30190,AT1G30250, AT1G30260, AT1G30475, AT1G30515, AT1G30757, AT1G30795,AT1G30814, AT1G30835, AT1G30845, AT1G30850, AT1G30880, AT1G31050,AT1G31060, AT1G31130, AT1G31150, AT1G31175, AT1G31200, AT1G31250,AT1G31270, AT1G31335, AT1G31460, AT1G31520, AT1G31580, AT1G31620,AT1G31750, AT1G31772, AT1G31835, AT1G31870, AT1G31940, AT1G31960,AT1G31990, AT1G32000, AT1G32010, AT1G32040, AT1G32260, AT1G32290,AT1G32310, AT1G32337, AT1G32370, AT1G32390, AT1G32460, AT1G32570,AT1G32583, AT1G32610, AT1G32630, AT1G32650, AT1G32670, AT1G32680,AT1G32690, AT1G32720, AT1G32810, AT1G32920, AT1G32928, AT1G32975,AT1G33055, AT1G33135, AT1G33490, AT1G33500, AT1G33607, AT1G33640,AT1G33700, AT1G33710, AT1G33810, AT1G33820, AT1G33850, AT1G33860,AT1G34000, AT1G34010, AT1G34047, AT1G34095, AT1G34245, AT1G34280,AT1G34315, AT1G34350, AT1G34400, AT1G34440, AT1G34490, AT1G34520,AT1G34550, AT1G34570, AT1G34590, AT1G34630, AT1G34640, AT1G34730,AT1G34770, AT1G34910, AT1G35030, AT1G35040, AT1G35080, AT1G35100,AT1G35183, AT1G35230, AT1G35320, AT1G35340, AT1G35375, AT1G35430,AT1G35435, AT1G35500, AT1G35513, AT1G35570, AT1G35614, AT1G35617,AT1G35640, AT1G35663, AT1G35780, AT1G35820, AT1G35880, AT1G35890,AT1G35900, AT1G36020, AT1G36100, AT1G36230, AT1G36320, AT1G36380,AT1G36395, AT1G36640, AT1G36670, AT1G36675, AT1G36745, AT1G36756,AT1G36763, AT1G36925, AT1G36940, AT1G36960, AT1G36970, AT1G36990,AT1G37000, AT1G37010, AT1G37015, AT1G37037, AT1G37045, AT1G38380,AT1G38630, AT1G38790, AT1G38950, AT1G39350, AT1G40080, AT1G40087,AT1G40115, AT1G40125, AT1G40129, AT1G40133, AT1G40230, AT1G41650,AT1G41750, AT1G41770, AT1G41810, AT1G41820, AT1G41855, AT1G41870,AT1G41900, AT1G41920, AT1G42080, AT1G42190, AT1G42367, AT1G42393,AT1G42430, AT1G42515, AT1G42550, AT1G42580, AT1G42630, AT1G42700,AT1G42710, AT1G42740, AT1G42960, AT1G43205, AT1G43230, AT1G43310,AT1G43320, AT1G43330, AT1G43415, AT1G43570, AT1G43580, AT1G43590,AT1G43660, AT1G43720, AT1G43730, AT1G43777, AT1G43790, AT1G43810,AT1G43850, AT1G43920, AT1G43940, AT1G43970, AT1G44000, AT1G44010,AT1G44085, AT1G44222, AT1G44414, AT1G44740, AT1G44770, AT1G44780,AT1G44850, AT1G44875, AT1G44920, AT1G44930, AT1G44960, AT1G44990,AT1G45150, AT1G45165, AT1G45170, AT1G45230, AT1G45233, AT1G45248,AT1G45403, AT1G45688, AT1G46336, AT1G47200, AT1G47280, AT1G47310,AT1G47317, AT1G47395, AT1G47400, AT1G47410, AT1G47420, AT1G47485,AT1G47495, AT1G47640, AT1G47660, AT1G47680, AT1G47690, AT1G47700,AT1G47770, AT1G47783, AT1G47813, AT1G47820, AT1G47940, AT1G47970,AT1G48145, AT1G48170, AT1G48200, AT1G48250, AT1G48280, AT1G48290,AT1G48325, AT1G48330, AT1G48360, AT1G48380, AT1G48440, AT1G48460,AT1G48510, AT1G48530, AT1G48580, AT1G48720, AT1G48730, AT1G48780,AT1G48840, AT1G49000, AT1G49005, AT1G49110, AT1G49140, AT1G49150,AT1G49260, AT1G49290, AT1G49310, AT1G49330, AT1G49410, AT1G49490,AT1G49500, AT1G49510, AT1G49680, AT1G49700, AT1G49715, AT1G49800,AT1G49840, AT1G49870, AT1G49930, AT1G49940, AT1G49975, AT1G50020,AT1G50080, AT1G50150, AT1G50220, AT1G50290, AT1G50350, AT1G50530,AT1G50660, AT1G50710, AT1G50730, AT1G50800, AT1G50910, AT1G50930,AT1G51000, AT1G51010, AT1G51030, AT1G51080, AT1G51100, AT1G51130,AT1G51355, AT1G51400, AT1G51405, AT1G51430, AT1G51840, AT1G51915,AT1G51920, AT1G51970, AT1G52080, AT1G52087, AT1G52090, AT1G52140,AT1G52155, AT1G52220, AT1G52270, AT1G52320, AT1G52390, AT1G52410,AT1G52430, AT1G52440, AT1G52450, AT1G52550, AT1G52565, AT1G52615,AT1G52680, AT1G52720, AT1G52780, AT1G52825, AT1G52827, AT1G52840,AT1G52855, AT1G52905, AT1G53035, AT1G53040, AT1G53180, AT1G53250,AT1G53260, AT1G53265, AT1G53285, AT1G53380, AT1G53400, AT1G53450,AT1G53460, AT1G53480, AT1G53490, AT1G53560, AT1G53610, AT1G53620,AT1G53625, AT1G53640, AT1G53645, AT1G53760, AT1G53770, AT1G53785,AT1G53800, AT1G53935, AT1G53970, AT1G54110, AT1G54120, AT1G54180,AT1G54200, AT1G54215, AT1G54217, AT1G54300, AT1G54420, AT1G54445,AT1G54470, AT1G54575, AT1G54640, AT1G54680, AT1G54700, AT1G54720,AT1G54730, AT1G54740, AT1G54770, AT1G54780, AT1G54840, AT1G54860,AT1G54880, AT1G54920, AT1G54923, AT1G54926, AT1G54950, AT1G54955,AT1G54970, AT1G55080, AT1G55160, AT1G55220, AT1G55250, AT1G55330,AT1G55365, AT1G55400, AT1G55475, AT1G55535, AT1G55540, AT1G55545,AT1G55675, AT1G55710, AT1G55800, AT1G55928, AT1G55990, AT1G56020,AT1G56060, AT1G56085, AT1G56100, AT1G56180, AT1G56200, AT1G56260,AT1G56270, AT1G56320, AT1G56415, AT1G56420, AT1G56530, AT1G56553,AT1G56555, AT1G56660, AT1G57540, AT1G57565, AT1G57670, AT1G57680,AT1G57760, AT1G58055, AT1G58120, AT1G58150, AT1G58225, AT1G58235,AT1G58242, AT1G58250, AT1G58330, AT1G58420, AT1G58460, AT1G58520,AT1G58766, AT1G59077, AT1G59510, AT1G59520, AT1G59535, AT1G59590,AT1G59600, AT1G59722, AT1G59835, AT1G59840, AT1G59865, AT1G59885,AT1G59920, AT1G59930, AT1G60000, AT1G60010, AT1G60060, AT1G60240,AT1G60250, AT1G60380, AT1G60460, AT1G60560, AT1G60610, AT1G60640,AT1G60720, AT1G60870, AT1G60983, AT1G60987, AT1G61000, AT1G61030,AT1G61080, AT1G61090, AT1G61095, AT1G61097, AT1G61100, AT1G61170,AT1G61200, AT1G61255, AT1G61340, AT1G61410, AT1G61450, AT1G61688,AT1G61780, AT1G61900, AT1G61920, AT1G62000, AT1G62045, AT1G62060,AT1G62070, AT1G62080, AT1G62190, AT1G62210, AT1G62220, AT1G62225,AT1G62240, AT1G62250, AT1G62440, AT1G62480, AT1G62690, AT1G62780,AT1G62855, AT1G62870, AT1G62890, AT1G62935, AT1G63055, AT1G63060,AT1G63105, AT1G63240, AT1G63245, AT1G63300, AT1G63310, AT1G63522,AT1G63530, AT1G63535, AT1G63540, AT1G63610, AT1G63670, AT1G63720,AT1G63960, AT1G64050, AT1G64080, AT1G64107, AT1G64140, AT1G64180,AT1G64295, AT1G64320, AT1G64330, AT1G64340, AT1G64355, AT1G64360,AT1G64370, AT1G64385, AT1G64405, AT1G64490, AT1G64560, AT1G64680,AT1G64690, AT1G64700, AT1G64800, AT1G64870, AT1G64990, AT1G65010,AT1G65090, AT1G65110, AT1G65120, AT1G65130, AT1G65200, AT1G65230,AT1G65270, AT1G65295, AT1G65342, AT1G65352, AT1G65470, AT1G65490,AT1G65500, AT1G65510, AT1G65710, AT1G65720, AT1G65845, AT1G66070,AT1G66080, AT1G66145, AT1G66190, AT1G66235, AT1G66245, AT1G66480,AT1G66790, AT1G66820, AT1G66840, AT1G66890, AT1G66940, AT1G67025,AT1G67035, AT1G67040, AT1G67050, AT1G67060, AT1G67080, AT1G67170,AT1G67195, AT1G67230, AT1G67350, AT1G67540, AT1G67635, AT1G67670,AT1G67700, AT1G67775, AT1G67790, AT1G67855, AT1G67860, AT1G67865,AT1G67870, AT1G67910, AT1G67920, AT1G67930, AT1G67950, AT1G68250,AT1G68330, AT1G68350, AT1G68430, AT1G68440, AT1G68490, AT1G68500,AT1G68680, AT1G68700, AT1G68725, AT1G68765, AT1G68790, AT1G68795,AT1G68845, AT1G68870, AT1G68875, AT1G68905, AT1G68907, AT1G68910,AT1G68935, AT1G68945, AT1G69050, AT1G69160, AT1G69170, AT1G69230,AT1G69280, AT1G69320, AT1G69380, AT1G69390, AT1G69430, AT1G69470,AT1G69760, AT1G69825, AT1G69935, AT1G69970, AT1G69980, AT1G70100,AT1G70160, AT1G70200, AT1G70220, AT1G70350, AT1G70470, AT1G70505,AT1G70620, AT1G70760, AT1G70770, AT1G70780, AT1G70895, AT1G70900,AT1G70985, AT1G70990, AT1G71015, AT1G71080, AT1G71110, AT1G71190,AT1G71235, AT1G71240, AT1G71310, AT1G71430, AT1G71470, AT1G71730,AT1G71740, AT1G71760, AT1G71780, AT1G71865, AT1G71910, AT1G71940,AT1G72020, AT1G72080, AT1G72240, AT1G72380, AT1G72390, AT1G72410,AT1G72420, AT1G72430, AT1G72490, AT1G72530, AT1G72580, AT1G72600,AT1G72645, AT1G72690, AT1G72720, AT1G72790, AT1G73060, AT1G73090,AT1G73120, AT1G73130, AT1G73165, AT1G73177, AT1G73240, AT1G73350,AT1G73470, AT1G73510, AT1G73603, AT1G73607, AT1G73770, AT1G73790,AT1G73840, AT1G73885, AT1G73940, AT1G73965, AT1G73970, AT1G74045,AT1G74055, AT1G74160, AT1G74220, AT1G74530, AT1G74860, AT1G74880,AT1G75060, AT1G75110, AT1G75150, AT1G75160, AT1G75180, AT1G75190,AT1G75260, AT1G75310, AT1G75360, AT1G75550, AT1G75730, AT1G75770,AT1G75810, AT1G75860, AT1G75870, AT1G76070, AT1G76185, AT1G76200,AT1G76230, AT1G76250, AT1G76340, AT1G76405, AT1G76450, AT1G76480,AT1G76600, AT1G76610, AT1G76660, AT1G76740, AT1G76780, AT1G76820,AT1G76840, AT1G76850, AT1G76910, AT1G76955, AT1G76960, AT1G76965,AT1G76980, AT1G77150, AT1G77270, AT1G77310, AT1G77350, AT1G77400,AT1G77500, AT1G77540, AT1G77655, AT1G77765, AT1G77855, AT1G77885,AT1G77890, AT1G77910, AT1G77960, AT1G78030, AT1G78070, AT1G78110,AT1G78150, AT1G78170, AT1G78650, AT1G78790, AT1G78810, AT1G78815,AT1G78880, AT1G78890, AT1G78895, AT1G78995, AT1G79020, AT1G79060,AT1G79070, AT1G79090, AT1G79100, AT1G79110, AT1G79160, AT1G79170,AT1G79200, AT1G79260, AT1G79390, AT1G79420, AT1G79430, AT1G79660,AT1G79830, AT1G79915, AT1G79970, AT1G79975, AT1G80000, AT1G80110,AT1G80180, AT1G80200, AT1G80210, AT1G80240, AT1G80245, AT1G80310,AT1G80540, AT1G80610, AT1G80700, AT1G80810, AT1G80860, AT1G80865,AT1G80890, AT1G80910, AT1G80940, AT1G80980, AT2G01031, AT2G01060,AT2G01100, AT2G01120, AT2G01175, AT2G01200, AT2G01300, AT2G01310,AT2G01340, AT2G01400, AT2G01505, AT2G01580, AT2G01590, AT2G01620,AT2G01640, AT2G01650, AT2G01670, AT2G01755, AT2G01800, AT2G01870,AT2G01913, AT2G01940, AT2G01960, AT2G01990, AT2G02070, AT2G02280,AT2G02350, AT2G02370, AT2G02440, AT2G02490, AT2G02510, AT2G02515,AT2G02520, AT2G02795, AT2G02835, AT2G02840, AT2G02880, AT2G02910,AT2G02950, AT2G03010, AT2G03070, AT2G03150, AT2G03180, AT2G03310,AT2G03320, AT2G03420, AT2G03440, AT2G03540, AT2G03570, AT2G03580,AT2G03630, AT2G03680, AT2G03810, AT2G03830, AT2G03932, AT2G03937,AT2G04000, AT2G04025, AT2G04034, AT2G04039, AT2G04045, AT2G04046,AT2G04063, AT2G04135, AT2G04235, AT2G04280, AT2G04305, AT2G04320,AT2G04340, AT2G04360, AT2G04370, AT2G04380, AT2G04410, AT2G04460,AT2G04480, AT2G04495, AT2G04515, AT2G04600, AT2G04675, AT2G04790,AT2G04795, AT2G04800, AT2G04870, AT2G04925, AT2G05000, AT2G05030,AT2G05117, AT2G05120, AT2G05185, AT2G05270, AT2G05290, AT2G05310,AT2G05350, AT2G05360, AT2G05370, AT2G05500, AT2G05564, AT2G05620,AT2G05645, AT2G05647, AT2G05752, AT2G05915, AT2G05950, AT2G06005,AT2G06010, AT2G06040, AT2G06095, AT2G06140, AT2G06166, AT2G06200,AT2G06230, AT2G06390, AT2G06420, AT2G06480, AT2G06555, AT2G06570,AT2G06620, AT2G06630, AT2G06645, AT2G06750, AT2G06775, AT2G06820,AT2G06906, AT2G06908, AT2G06914, AT2G07000, AT2G07190, AT2G07215,AT2G07280, AT2G07290, AT2G07310, AT2G07440, AT2G07505, AT2G07520,AT2G07669, AT2G07672, AT2G07673, AT2G07674, AT2G07676, AT2G07678,AT2G07679, AT2G07691, AT2G07692, AT2G07701, AT2G07702, AT2G07705,AT2G07706, AT2G07708, AT2G07710, AT2G07713, AT2G07714, AT2G07719,AT2G07721, AT2G07722, AT2G07724, AT2G07728, AT2G07738, AT2G07760,AT2G07772, AT2G07773, AT2G07774, AT2G07775, AT2G07776, AT2G07777,AT2G07779, AT2G07787, AT2G07795, AT2G07880, AT2G07981, AT2G08986,AT2G09388, AT2G09840, AT2G09865, AT2G09900, AT2G10020, AT2G10070,AT2G10090, AT2G10105, AT2G10110, AT2G10175, AT2G10285, AT2G10340,AT2G10360, AT2G10380, AT2G10390, AT2G10470, AT2G10550, AT2G10555,AT2G10560, AT2G10602, AT2G10608, AT2G10850, AT2G10870, AT2G10920,AT2G10930, AT2G10965, AT2G10975, AT2G10980, AT2G11005, AT2G11015,AT2G11090, AT2G11135, AT2G11370, AT2G11405, AT2G11462, AT2G11570,AT2G11620, AT2G11626, AT2G11775, AT2G11910, AT2G12110, AT2G12120,AT2G12130, AT2G12170, AT2G12290, AT2G12320, AT2G12400, AT2G12405,AT2G12465, AT2G12475, AT2G12505, AT2G12610, AT2G12685, AT2G12700,AT2G12875, AT2G12905, AT2G12935, AT2G12945, AT2G13125, AT2G13126,AT2G13270, AT2G13320, AT2G13430, AT2G13450, AT2G13500, AT2G13510,AT2G13550, AT2G13650, AT2G13660, AT2G13690, AT2G13730, AT2G13760,AT2G13770, AT2G13865, AT2G13975, AT2G14000, AT2G14020, AT2G14045,AT2G14095, AT2G14240, AT2G14247, AT2G14340, AT2G14390, AT2G14460,AT2G14590, AT2G14600, AT2G14635, AT2G14680, AT2G14700, AT2G14730,AT2G14760, AT2G14774, AT2G14800, AT2G14810, AT2G14850, AT2G14890,AT2G14910, AT2G14935, AT2G15000, AT2G15020, AT2G15185, AT2G15290,AT2G15327, AT2G15340, AT2G15345, AT2G15420, AT2G15500, AT2G15520,AT2G15535, AT2G15550, AT2G15600, AT2G15670, AT2G15800, AT2G15815,AT2G15830, AT2G15860, AT2G15880, AT2G15890, AT2G15930, AT2G15960,AT2G16015, AT2G16020, AT2G16070, AT2G16170, AT2G16190, AT2G16200,AT2G16270, AT2G16340, AT2G16365, AT2G16385, AT2G16410, AT2G16485,AT2G16575, AT2G16586, AT2G16595, AT2G16630, AT2G16676, AT2G16820,AT2G17110, AT2G17160, AT2G17240, AT2G17300, AT2G17320, AT2G17340,AT2G17350, AT2G17442, AT2G17540, AT2G17550, AT2G17695, AT2G17710,AT2G17723, AT2G17780, AT2G17785, AT2G17787, AT2G17960, AT2G17972,AT2G17990, AT2G18070, AT2G18200, AT2G18210, AT2G18240, AT2G18270,AT2G18410, AT2G18440, AT2G18610, AT2G18680, AT2G18690, AT2G18830,AT2G18870, AT2G18876, AT2G18910, AT2G18920, AT2G18930, AT2G18970,AT2G19000, AT2G19090, AT2G19180, AT2G19200, AT2G19220, AT2G19270,AT2G19290, AT2G19300, AT2G19320, AT2G19340, AT2G19390, AT2G19420,AT2G19460, AT2G19530, AT2G19700, AT2G19802, AT2G19850, AT2G19893,AT2G19950, AT2G20080, AT2G20142, AT2G20150, AT2G20208, AT2G20230,AT2G20240, AT2G20250, AT2G20310, AT2G20390, AT2G20410, AT2G20463,AT2G20480, AT2G20495, AT2G20500, AT2G20515, AT2G20585, AT2G20590,AT2G20595, AT2G20616, AT2G20620, AT2G20625, AT2G20700, AT2G20740,AT2G20760, AT2G20820, AT2G20825, AT2G20835, AT2G20870, AT2G20875,AT2G20890, AT2G20920, AT2G20970, AT2G20980, AT2G21080, AT2G21180,AT2G21185, AT2G21195, AT2G21237, AT2G21290, AT2G21385, AT2G21465,AT2G21560, AT2G21640, AT2G21660, AT2G21720, AT2G21725, AT2G21780,AT2G21800, AT2G21820, AT2G21870, AT2G21960, AT2G21970, AT2G21980,AT2G22000, AT2G22080, AT2G22121, AT2G22122, AT2G22140, AT2G22270,AT2G22320, AT2G22340, AT2G22470, AT2G22510, AT2G22520, AT2G22560,AT2G22790, AT2G22795, AT2G22805, AT2G22807, AT2G22820, AT2G22840,AT2G22890, AT2G22905, AT2G22940, AT2G22941, AT2G23040, AT2G23090,AT2G23093, AT2G23110, AT2G23120, AT2G23130, AT2G23270, AT2G23370,AT2G23390, AT2G23440, AT2G23490, AT2G23530, AT2G23670, AT2G23690,AT2G23755, AT2G23920, AT2G23985, AT2G24100, AT2G24285, AT2G24310,AT2G24330, AT2G24340, AT2G24410, AT2G24440, AT2G24460, AT2G24550,AT2G24617, AT2G24625, AT2G24762, AT2G24780, AT2G24910, AT2G24945,AT2G24960, AT2G24970, AT2G25185, AT2G25250, AT2G25260, AT2G25270,AT2G25510, AT2G25565, AT2G25605, AT2G25625, AT2G25670, AT2G25680,AT2G25685, AT2G25720, AT2G25735, AT2G25920, AT2G25930, AT2G25990,AT2G26110, AT2G26120, AT2G26340, AT2G26520, AT2G26770, AT2G26810,AT2G26840, AT2G26880, AT2G27090, AT2G27100, AT2G27180, AT2G27250,AT2G27280, AT2G27285, AT2G27315, AT2G27380, AT2G27385, AT2G27390,AT2G27402, AT2G27535, AT2G27540, AT2G27630, AT2G27650, AT2G27730,AT2G27775, AT2G27830, AT2G27840, AT2G27950, AT2G28020, AT2G28130,AT2G28230, 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AT2G33855, AT2G34010, AT2G34100,AT2G34110, AT2G34120, AT2G34123, AT2G34150, AT2G34185, AT2G34220,AT2G34230, AT2G34240, AT2G34270, AT2G34310, AT2G34315, AT2G34330,AT2G34510, AT2G34530, AT2G34580, AT2G34585, AT2G34640, AT2G34655,AT2G34670, AT2G34690, AT2G34730, AT2G34780, AT2G34800, AT2G34870,AT2G34910, AT2G35070, AT2G35075, AT2G35080, AT2G35090, AT2G35110,AT2G35200, AT2G35215, AT2G35230, AT2G35260, AT2G35290, AT2G35470,AT2G35480, AT2G35585, AT2G35612, AT2G35670, AT2G35710, AT2G35733,AT2G35736, AT2G35750, AT2G35790, AT2G35810, AT2G35820, AT2G35830,AT2G35850, AT2G35870, AT2G35900, AT2G35950, AT2G36030, AT2G36040,AT2G36145, AT2G36220, AT2G36255, AT2G36295, AT2G36400, AT2G36420,AT2G36440, AT2G36485, AT2G36550, AT2G36695, AT2G36724, AT2G36835,AT2G36885, AT2G36895, AT2G36920, AT2G36940, AT2G37035, AT2G37070,AT2G37080, AT2G37100, AT2G37195, AT2G37300, AT2G37370, AT2G37380,AT2G37530, AT2G37610, AT2G37680, AT2G37750, AT2G37860, AT2G37910,AT2G37920, AT2G37975, AT2G38090, AT2G38140, AT2G38160, AT2G38220,AT2G38350, AT2G38430, AT2G38440, AT2G38465, AT2G38570, AT2G38580,AT2G38690, AT2G38695, AT2G38790, AT2G38823, AT2G38890, AT2G39000,AT2G39160, AT2G39170, AT2G39200, AT2G39300, AT2G39370, AT2G39500,AT2G39520, AT2G39560, AT2G39680, AT2G39855, AT2G39870, AT2G39950,AT2G40020, AT2G40060, AT2G40070, AT2G40085, AT2G40095, AT2G40113,AT2G40316, AT2G40390, AT2G40410, AT2G40475, AT2G40530, AT2G40550,AT2G40630, AT2G40680, AT2G40765, AT2G40955, AT2G41150, AT2G41230,AT2G41260, AT2G41280, AT2G41350, AT2G41390, AT2G41400, AT2G41420,AT2G41440, AT2G41570, AT2G41610, AT2G41650, AT2G41730, AT2G41760,AT2G41770, AT2G41780, AT2G41800, AT2G41810, AT2G41905, AT2G41945,AT2G41960, AT2G41990, AT2G42040, AT2G42050, AT2G42130, AT2G42180,AT2G42190, AT2G42260, AT2G42280, AT2G42310, AT2G42320, AT2G42340,AT2G42370, AT2G42395, AT2G42540, AT2G42610, AT2G42640, AT2G42760,AT2G42840, AT2G42860, AT2G42900, AT2G42950, AT2G42955, AT2G42975,AT2G43110, AT2G43250, AT2G43390, AT2G43450, AT2G43540, AT2G43630,AT2G43780, AT2G43795, AT2G43945, AT2G43950, AT2G43990, AT2G44010,AT2G44080, AT2G44195, AT2G44200, AT2G44280, AT2G44420, AT2G44510,AT2G44600, AT2G44640, AT2G44735, AT2G44740, AT2G44760, AT2G44820,AT2G44850, AT2G45000, AT2G45060, AT2G45250, AT2G45260, AT2G45380,AT2G45403, AT2G45450, AT2G45480, AT2G45520, AT2G45690, AT2G45780,AT2G45860, AT2G45900, AT2G45930, AT2G45980, AT2G46000, AT2G46060,AT2G46080, AT2G46180, AT2G46200, AT2G46250, AT2G46360, AT2G46375,AT2G46380, AT2G46390, AT2G46455, AT2G46490, AT2G46540, AT2G46550,AT2G46640, AT2G46735, AT2G46820, AT2G46830, AT2G46915, AT2G46940,AT2G46980, AT2G47010, AT2G47020, AT2G47115, AT2G47200, AT2G47360,AT2G47480, AT2G47485, AT2G47530, AT2G47660, AT2G47690, AT2G47720,AT2G47840, AT2G47910, AT2G47930, AT2G47950, AT2G48040, AT2G48050,AT2G48060, AT2G48070, AT2G48075, AT2G48090, AT2G48120, AT3G01060,AT3G01130, AT3G01160, AT3G01170, AT3G01230, AT3G01240, AT3G01250,AT3G01323, AT3G01325, AT3G01345, AT3G01430, AT3G01435, AT3G01513,AT3G01516, AT3G01670, AT3G01680, AT3G01700, AT3G01710, AT3G01720,AT3G01730, AT3G01740, AT3G01810, AT3G01860, AT3G01940, AT3G01950,AT3G01960, AT3G02120, AT3G02125, AT3G02170, AT3G02180, AT3G02220,AT3G02240, AT3G02370, AT3G02390, AT3G02420, AT3G02500, AT3G02555,AT3G02560, AT3G02640, AT3G02670, AT3G02680, AT3G02790, AT3G02860,AT3G02900, AT3G02930, AT3G03020, AT3G03070, AT3G03130, AT3G03150,AT3G03160, AT3G03170, AT3G03210, AT3G03420, AT3G03460, AT3G03560,AT3G03570, AT3G03770, AT3G03773, AT3G03870, AT3G03930, AT3G04020,AT3G04040, AT3G04160, AT3G04310, AT3G04510, AT3G04550, AT3G04560,AT3G04640, AT3G04740, AT3G04903, AT3G04943, AT3G04945, AT3G04960,AT3G04990, AT3G05010, AT3G05020, AT3G05080, AT3G05110, AT3G05130,AT3G05220, AT3G05320, AT3G05330, AT3G05410, AT3G05460, AT3G05550,AT3G05570, AT3G05725, AT3G05727, AT3G05730, AT3G05830, AT3G05900,AT3G05935, AT3G05937, AT3G05980, AT3G06020, AT3G06070, AT3G06090,AT3G06145, AT3G06180, AT3G06360, AT3G06435, AT3G06545, AT3G06547,AT3G06600, AT3G06610, AT3G06670, AT3G06710, AT3G06750, AT3G06780,AT3G06790, AT3G06840, AT3G06870, AT3G06890, AT3G06895, AT3G06960,AT3G07005, AT3G07150, AT3G07180, AT3G07195, AT3G07210, AT3G07425,AT3G07440, AT3G07510, AT3G07560, AT3G07568, AT3G07580, AT3G07640,AT3G07710, AT3G07730, AT3G07790, AT3G07910, AT3G07950, AT3G08030,AT3G08490, AT3G08550, AT3G08610, AT3G08630, AT3G08640, AT3G08650,AT3G08670, AT3G08780, AT3G08880, AT3G08955, AT3G09000, AT3G09032,AT3G09050, AT3G09085, AT3G09130, AT3G09162, AT3G09180, AT3G09280,AT3G09430, AT3G09450, AT3G09730, AT3G09750, AT3G09770, AT3G09860,AT3G09922, AT3G09950, AT3G10020, AT3G10116, AT3G10120, AT3G10195,AT3G10525, AT3G10572, AT3G10650, AT3G10810, AT3G10830, AT3G10880,AT3G10930, AT3G10980, AT3G11030, AT3G11060, AT3G11160, AT3G11300,AT3G11310, AT3G11325, AT3G11405, AT3G11560, AT3G11590, AT3G11600,AT3G11640, AT3G11670, AT3G11690, AT3G11745, AT3G11760, AT3G11800,AT3G11810, AT3G11860, AT3G11880, AT3G12190, AT3G12210, AT3G12320,AT3G12345, AT3G12510, AT3G12650, AT3G12840, AT3G12870, AT3G12910,AT3G12920, AT3G12955, AT3G12960, AT3G12970, AT3G12977, AT3G13130,AT3G13175, AT3G13227, AT3G13240, AT3G13275, AT3G13360, AT3G13370,AT3G13403, AT3G13410, AT3G13420, AT3G13435, AT3G13460, AT3G13480,AT3G13500, AT3G13520, AT3G13630, AT3G13674, AT3G13677, AT3G13780,AT3G13845, AT3G13857, AT3G13910, AT3G13950, AT3G13960, AT3G13980,AT3G14060, AT3G14190, AT3G14280, AT3G14340, AT3G14395, AT3G14430,AT3G14480, AT3G14560, AT3G14595, AT3G14670, AT3G14750, AT3G14760,AT3G14780, AT3G14830, AT3G14840, AT3G14870, AT3G14880, AT3G14900,AT3G14910, AT3G14920, AT3G15000, AT3G15095, AT3G15110, AT3G15115,AT3G15230, AT3G15240, AT3G15250, AT3G15280, AT3G15310, AT3G15340,AT3G15351, AT3G15357, AT3G15358, AT3G15395, AT3G15400, AT3G15420,AT3G15440, AT3G15550, AT3G15560, AT3G15630, AT3G15750, AT3G15760,AT3G15770, AT3G15780, AT3G15820, AT3G15830, AT3G15840, AT3G15860,AT3G15900, AT3G15910, AT3G15950, AT3G16000, AT3G16040, AT3G16070,AT3G16200, AT3G16220, AT3G16330, AT3G16660, AT3G16670, AT3G16750,AT3G16895, AT3G16930, AT3G17120, AT3G17155, AT3G17160, AT3G17190,AT3G17350, AT3G17460, AT3G17580, AT3G17780, AT3G17890, AT3G17900,AT3G17930, AT3G17950, AT3G18050, AT3G18240, AT3G18250, AT3G18300,AT3G18310, AT3G18350, AT3G18410, AT3G18485, AT3G18510, AT3G18540,AT3G18560, AT3G18700, AT3G18770, AT3G18800, AT3G18940, AT3G19020,AT3G19030, AT3G19055, AT3G19120, AT3G19180, AT3G19200, AT3G19220,AT3G19250, AT3G19330, AT3G19340, AT3G19530, AT3G19540, AT3G19550,AT3G19650, AT3G19660, AT3G19750, AT3G19790, AT3G19800, AT3G19900,AT3G19920, AT3G20070, AT3G20155, AT3G20340, AT3G20350, AT3G20362,AT3G20430, AT3G20450, AT3G20490, AT3G20555, AT3G20557, AT3G20680,AT3G20720, AT3G20760, AT3G20850, AT3G20865, AT3G20900, AT3G20920,AT3G20980, AT3G21000, AT3G21055, AT3G21080, AT3G21260, AT3G21290,AT3G21320, AT3G21400, AT3G21465, AT3G21570, AT3G21680, AT3G21710,AT3G21865, AT3G22070, AT3G22090, AT3G22210, AT3G22231, AT3G22235,AT3G22240, AT3G22270, AT3G22380, AT3G22415, AT3G22430, AT3G22510,AT3G22680, AT3G22790, AT3G22820, AT3G22840, AT3G22942, AT3G23040,AT3G23165, AT3G23167, AT3G23170, AT3G23172, AT3G23245, AT3G23290,AT3G23295, AT3G23440, AT3G23590, AT3G23650, AT3G23715, AT3G23720,AT3G23727, AT3G23740, AT3G23850, AT3G23860, AT3G23910, AT3G24160,AT3G24180, AT3G24225, AT3G24250, AT3G24255, AT3G24280, AT3G24380,AT3G24490, AT3G24506, AT3G24508, AT3G24510, AT3G24513, AT3G24517,AT3G24535, AT3G24630, AT3G24640, AT3G24680, AT3G24690, AT3G24750,AT3G24770, AT3G24780, AT3G25080, AT3G25130, AT3G25200, AT3G25400,AT3G25545, AT3G25590, AT3G25597, AT3G25640, AT3G25655, AT3G25690,AT3G25720, AT3G25727, AT3G25805, AT3G25870, AT3G25882, AT3G25905,AT3G26000, AT3G26110, AT3G26235, AT3G26616, AT3G26710, AT3G26750,AT3G26800, AT3G26850, AT3G26855, AT3G26890, AT3G26910, AT3G26950,AT3G26960, AT3G27025, AT3G27030, AT3G27050, AT3G27100, AT3G27130,AT3G27210, AT3G27250, AT3G27350, AT3G27370, AT3G27390, AT3G27420,AT3G27520, AT3G27590, AT3G27630, AT3G27750, AT3G27770, AT3G27800,AT3G27906, AT3G27930, AT3G27990, AT3G28020, AT3G28110, AT3G28120,AT3G28155, AT3G28170, AT3G28190, AT3G28240, AT3G28260, AT3G28280,AT3G28350, AT3G28370, AT3G28420, AT3G28455, AT3G28530, AT3G28560,AT3G28590, AT3G28670, AT3G28720, AT3G28770, AT3G29010, AT3G29033,AT3G29034, AT3G29075, AT3G29080, AT3G29130, AT3G29140, AT3G29185,AT3G29210, AT3G29220, AT3G29265, AT3G29280, AT3G29300, AT3G29305,AT3G29385, AT3G29420, AT3G29450, AT3G29470, AT3G29560, AT3G29570,AT3G29600, AT3G29610, AT3G29636, AT3G29700, AT3G29763, AT3G29785,AT3G29786, AT3G29790, AT3G29796, AT3G30150, AT3G30160, AT3G30190,AT3G30200, AT3G30220, AT3G30250, AT3G30320, AT3G30350, AT3G30360,AT3G30370, AT3G30490, AT3G30510, AT3G30520, AT3G30580, AT3G30590,AT3G30610, AT3G30645, AT3G30650, AT3G30660, AT3G30670, AT3G30690,AT3G30700, AT3G30720, AT3G30725, AT3G30730, AT3G30750, AT3G30751,AT3G30755, AT3G30816, AT3G30820, AT3G30840, AT3G30843, AT3G30845,AT3G30848, AT3G31300, AT3G31310, AT3G31320, AT3G31330, AT3G31350,AT3G31370, AT3G31400, AT3G31406, AT3G31430, AT3G31540, AT3G31900,AT3G31910, AT3G31915, AT3G31940, AT3G31955, AT3G32050, AT3G32070,AT3G32100, AT3G32120, AT3G32150, AT3G32160, AT3G32180, AT3G32190,AT3G32200, AT3G32330, AT3G32410, AT3G32896, AT3G32902, AT3G32903,AT3G32904, AT3G32930, AT3G32960, AT3G33064, AT3G33073, AT3G33080,AT3G33131, AT3G33187, AT3G33230, AT3G33293, AT3G33393, AT3G33448,AT3G33494, AT3G33572, AT3G42070, AT3G42090, AT3G42120, AT3G42130,AT3G42140, AT3G42190, AT3G42200, AT3G42240, AT3G42250, AT3G42254,AT3G42300, AT3G42310, AT3G42320, AT3G42350, AT3G42380, AT3G42390,AT3G42430, AT3G42436, AT3G42473, AT3G42480, AT3G42490, AT3G42510,AT3G42520, AT3G42540, AT3G42556, AT3G42560, AT3G42590, AT3G42610,AT3G42680, AT3G42700, AT3G42723, AT3G42725, AT3G42740, AT3G42750,AT3G42780, AT3G42786, AT3G42800, AT3G42810, AT3G42870, AT3G42920,AT3G42970, AT3G42990, AT3G43110, AT3G43140, AT3G43150, AT3G43153,AT3G43160, AT3G43260, AT3G43280, AT3G43290, AT3G43330, AT3G43410,AT3G43420, AT3G43450, AT3G43470, AT3G43480, AT3G43500, AT3G43528,AT3G43580, AT3G43583, AT3G43680, AT3G43682, AT3G43760, AT3G43770,AT3G43833, AT3G43850, AT3G43863, AT3G43870, AT3G43880, AT3G43900,AT3G43910, AT3G43930, AT3G43940, AT3G43970, AT3G44020, AT3G44040,AT3G44070, AT3G44115, AT3G44140, AT3G44150, AT3G44170, AT3G44210,AT3G44230, AT3G44235, AT3G44280, AT3G44370, AT3G44430, AT3G44440,AT3G44450, AT3G44470, AT3G44570, AT3G44580, AT3G44690, AT3G44716,AT3G44750, AT3G44755, AT3G44760, AT3G44770, AT3G44935, AT3G44950,AT3G44960, AT3G44980, AT3G45040, AT3G45050, AT3G45093, AT3G45110,AT3G45120, AT3G45160, AT3G45200, AT3G45230, AT3G45320, AT3G45360,AT3G45370, AT3G45443, AT3G45577, AT3G45730, AT3G45755, AT3G45820,AT3G45830, AT3G45900, AT3G45910, AT3G46150, AT3G46220, AT3G46240,AT3G46270, AT3G46300, AT3G46310, AT3G46360, AT3G46380, AT3G46390,AT3G46430, AT3G46630, AT3G46750, AT3G46880, AT3G46890, AT3G47070,AT3G47100, AT3G47230, AT3G47240, AT3G47295, AT3G47320, AT3G47410,AT3G47490, AT3G47510, AT3G47630, AT3G47675, AT3G47833, AT3G47836,AT3G47850, AT3G47920, AT3G47965, AT3G48020, AT3G48120, AT3G48180,AT3G48185, AT3G48200, AT3G48220, AT3G48231, AT3G48470, AT3G48490,AT3G48510, AT3G48550, AT3G48630, AT3G48640, AT3G48660, AT3G48675,AT3G48710, AT3G48860, AT3G49055, AT3G49230, AT3G49250, AT3G49270,AT3G49280, AT3G49290, AT3G49300, AT3G49305, AT3G49307, AT3G49410,AT3G49460, AT3G49490, AT3G49540, AT3G49550, AT3G49570, AT3G49580,AT3G49590, AT3G49770, AT3G49790, AT3G49820, AT3G49840, AT3G49890,AT3G49990, AT3G50040, AT3G50250, AT3G50320, AT3G50340, AT3G50370,AT3G50373, AT3G50376, AT3G50430, AT3G50540, AT3G50550, AT3G50570,AT3G50580, AT3G50610, AT3G50640, AT3G50685, AT3G50800, AT3G50900,AT3G50910, AT3G50925, AT3G51010, AT3G51100, AT3G51230, AT3G51290,AT3G51350, AT3G51500, AT3G51510, AT3G51540, AT3G51580, AT3G51610,AT3G51640, AT3G51650, AT3G51750, AT3G51890, AT3G51970, AT3G52040,AT3G52070, AT3G52105, AT3G52110, AT3G52115, AT3G52170, AT3G52220,AT3G52230, AT3G52240, AT3G52360, AT3G52420, AT3G52480, AT3G52520,AT3G52540, AT3G52550, AT3G52620, AT3G52700, AT3G52710, AT3G52740,AT3G52770, AT3G52860, AT3G53235, AT3G53270, AT3G53320, AT3G53350,AT3G53470, AT3G53540, AT3G53630, AT3G53670, AT3G53860, AT3G53970,AT3G54000, AT3G54060, AT3G54170, AT3G54310, AT3G54500, AT3G54520,AT3G54530, AT3G54630, AT3G54680, AT3G54710, AT3G54730, AT3G54750,AT3G54880, AT3G55060, AT3G55160, AT3G55250, AT3G55420, AT3G55570,AT3G55600, AT3G55646, AT3G55690, AT3G55720, AT3G55760, AT3G55790,AT3G55860, AT3G55880, AT3G55910, AT3G55930, AT3G56010, AT3G56160,AT3G56220, AT3G56250, AT3G56260, AT3G56290, AT3G56360, AT3G56390,AT3G56410, AT3G56430, AT3G56480, AT3G56500, AT3G56590, AT3G56610,AT3G56650, AT3G56670, AT3G56720, AT3G56750, AT3G56790, AT3G56870,AT3G56910, AT3G57110, AT3G57160, AT3G57200, AT3G57210, AT3G57320,AT3G57360, AT3G57400, AT3G57420, AT3G57440, AT3G57450, AT3G57500,AT3G57690, AT3G57780, AT3G57785, AT3G57850, AT3G57860, AT3G57930,AT3G57950, AT3G57990, AT3G58010, AT3G58020, AT3G58050, AT3G58080,AT3G58110, AT3G58230, AT3G58280, AT3G58300, AT3G58320, AT3G58330,AT3G58470, AT3G58480, AT3G58540, AT3G58770, AT3G58840, AT3G58870,AT3G59090, AT3G59300, AT3G59370, AT3G59390, AT3G59430, AT3G59460,AT3G59490, AT3G59640, AT3G59680, AT3G59800, AT3G59840, AT3G59870,AT3G59880, AT3G59900, AT3G59930, AT3G60070, AT3G60200, AT3G60230,AT3G60310, AT3G60320, AT3G60380, AT3G60480, AT3G60520, AT3G60560,AT3G60590, AT3G60650, AT3G60680, AT3G60760, AT3G60850, AT3G60890,AT3G60930, AT3G61370, AT3G61380, AT3G61500, AT3G61570, AT3G61660,AT3G61670, AT3G61710, AT3G61780, AT3G61840, AT3G61870, AT3G61898,AT3G61920, AT3G61930, AT3G62070, AT3G62140, AT3G62320, AT3G62350,AT3G62370, AT3G62380, AT3G62400, AT3G62450, AT3G62480, AT3G62490,AT3G62500, AT3G62510, AT3G62580, AT3G62640, AT3G62650, AT3G62680,AT3G62730, AT3G62790, AT3G62920, AT3G62990, AT3G63020, AT3G63040,AT3G63050, AT3G63100, AT3G63160, AT3G63180, AT3G63270, AT3G63420,AT3G63430, AT4G00280, AT4G00310, AT4G00355, AT4G00440, AT4G00450,AT4G00525, AT4G00530, AT4G00580, AT4G00585, AT4G00695, AT4G00770,AT4G00890, AT4G00920, AT4G00930, AT4G00950, AT4G00955, AT4G01090,AT4G01150, AT4G01170, AT4G01245, AT4G01290, AT4G01340, AT4G01360,AT4G01500, AT4G01525, AT4G01530, AT4G01535, AT4G01590, AT4G01670,AT4G01735, AT4G01895, AT4G01915, AT4G01935, AT4G01960, AT4G01985,AT4G01995, AT4G02000, AT4G02030, AT4G02040, AT4G02090, AT4G02140,AT4G02160, AT4G02170, AT4G02210, AT4G02270, AT4G02425, AT4G02465,AT4G02482, AT4G02530, AT4G02550, AT4G02715, AT4G02725, AT4G02760,AT4G02800, AT4G02810, AT4G02830, AT4G02870, AT4G02880, AT4G02920,AT4G03090, AT4G03150, AT4G03165, AT4G03180, AT4G03305, AT4G03380,AT4G03505, AT4G03580, AT4G03600, AT4G03620, AT4G03680, AT4G03740,AT4G03750, AT4G03820, AT4G03940, AT4G03970, AT4G03975, AT4G03979,AT4G04030, AT4G04155, AT4G04190, AT4G04273, AT4G04330, AT4G04394,AT4G04396, AT4G04398, AT4G04423, AT4G04470, AT4G04480, AT4G04525,AT4G04614, AT4G04635, AT4G04650, AT4G04730, AT4G04745, AT4G04820,AT4G04925, AT4G04980, AT4G05070, AT4G05095, AT4G05145, AT4G05290,AT4G05370, AT4G05400, AT4G05523, AT4G05553, AT4G05560, AT4G05580,AT4G05581, AT4G05616, AT4G05631, AT4G05632, AT4G05636, AT4G05640,AT4G06490, AT4G06534, AT4G06603, AT4G06637, AT4G06672, AT4G06676,AT4G06716, AT4G06724, AT4G06728, AT4G06735, AT4G06740, AT4G07380,AT4G07452, AT4G07460, AT4G07485, AT4G07500, AT4G07515, AT4G07523,AT4G07524, AT4G07526, AT4G07666, AT4G07675, AT4G07740, AT4G07800,AT4G07825, AT4G07868, AT4G07932, AT4G07940, AT4G07943, AT4G07965,AT4G08013, AT4G08025, AT4G08028, AT4G08039, AT4G08056, AT4G08097,AT4G08098, AT4G08111, AT4G08130, AT4G08140, AT4G08200, AT4G08230,AT4G08240, AT4G08270, AT4G08310, AT4G08330, AT4G08336, AT4G08395,AT4G08485, AT4G08510, AT4G08540, AT4G08555, AT4G08593, AT4G08602,AT4G08630, AT4G08710, AT4G08730, AT4G08740, AT4G08760, AT4G08810,AT4G08820, AT4G08868, AT4G08869, AT4G08874, AT4G08875, AT4G08876,AT4G08895, AT4G08910, AT4G09030, AT4G09060, AT4G09070, AT4G09153,AT4G09170, AT4G09210, AT4G09220, AT4G09260, AT4G09270, AT4G09290,AT4G09390, AT4G09550, AT4G09580, AT4G09630, AT4G09647, AT4G09680,AT4G09700, AT4G09840, AT4G09850, AT4G09860, AT4G09880, AT4G09890,AT4G09965, AT4G09970, AT4G09984, AT4G10060, AT4G10080, AT4G10090,AT4G10140, AT4G10180, AT4G10230, AT4G10265, AT4G10270, AT4G10330,AT4G10613, AT4G10660, AT4G10670, AT4G10700, AT4G10800, AT4G10810,AT4G10820, AT4G10845, AT4G10860, AT4G10870, AT4G10890, AT4G10910,AT4G10930, AT4G10970, AT4G11020, AT4G11100, AT4G11211, AT4G11385,AT4G11393, AT4G11430, AT4G11700, AT4G11720, AT4G11780, AT4G11870,AT4G11910, AT4G11940, AT4G11990, AT4G12000, AT4G12005, AT4G12070,AT4G12220, AT4G12350, AT4G12370, AT4G12380, AT4G12540, AT4G12580,AT4G12680, AT4G12700, AT4G12735, AT4G12760, AT4G12770, AT4G12780,AT4G12930, AT4G12940, AT4G12970, AT4G12990, AT4G13095, AT4G13140,AT4G13150, AT4G13195, AT4G13200, AT4G13220, AT4G13235, AT4G13266,AT4G13270, AT4G13320, AT4G13340, AT4G13470, AT4G13500, AT4G13520,AT4G13530, AT4G13540, AT4G13690, AT4G13740, AT4G13955, AT4G13968,AT4G14100, AT4G14104, AT4G14120, AT4G14200, AT4G14270, AT4G14272,AT4G14276, AT4G14315, AT4G14380, AT4G14385, AT4G14450, AT4G14530,AT4G14590, AT4G14615, AT4G14650, AT4G14690, AT4G14723, AT4G14760,AT4G14810, AT4G14950, AT4G14970, AT4G14990, AT4G15030, AT4G15096,AT4G15140, AT4G15150, AT4G15160, AT4G15460, AT4G15540, AT4G15563,AT4G15640, AT4G15650, AT4G15710, AT4G15733, AT4G15735, AT4G15820,AT4G15885, AT4G15950, AT4G15970, AT4G15990, AT4G16000, AT4G16040,AT4G16060, AT4G16090, AT4G16140, AT4G16170, AT4G16215, AT4G16240,AT4G16320, AT4G16400, AT4G16444, AT4G16447, AT4G16450, AT4G16460,AT4G16515, AT4G16695, AT4G16810, AT4G16840, AT4G16845, AT4G16850,AT4G16980, AT4G17000, AT4G17010, AT4G17110, AT4G17130, AT4G17215,AT4G17240, AT4G17250, AT4G17310, AT4G17430, AT4G17540, AT4G17590,AT4G17600, AT4G17620, AT4G17700, AT4G17713, AT4G17790, AT4G17930,AT4G17960, AT4G17990, AT4G18000, AT4G18070, AT4G18080, AT4G18090,AT4G18140, AT4G18280, AT4G18310, AT4G18320, AT4G18335, AT4G18395,AT4G18400, AT4G18420, AT4G18470, AT4G18490, AT4G18500, AT4G18501,AT4G18510, AT4G18540, AT4G18570, AT4G18580, AT4G18593, AT4G18600,AT4G18610, AT4G18650, AT4G18660, AT4G18670, AT4G18680, AT4G18690,AT4G18823, AT4G18830, AT4G18850, AT4G18860, AT4G19070, AT4G19095,AT4G19100, AT4G19140, AT4G19160, AT4G19190, AT4G19200, AT4G19240,AT4G19270, AT4G19280, AT4G19290, AT4G19305, AT4G19320, AT4G19350,AT4G19360, AT4G19430, AT4G19480, AT4G19620, AT4G19905, AT4G19950,AT4G19980, AT4G20020, AT4G20095, AT4G20150, AT4G20160, AT4G20190,AT4G20250, AT4G20290, AT4G20330, AT4G20350, AT4G20390, AT4G20470,AT4G20500, AT4G20510, AT4G20690, AT4G20715, AT4G20720, AT4G20880,AT4G21010, AT4G21105, AT4G21140, AT4G21215, AT4G21445, AT4G21460,AT4G21500, AT4G21620, AT4G21700, AT4G21720, AT4G21740, AT4G21780,AT4G21865, AT4G21890, AT4G21920, AT4G21950, AT4G22000, AT4G22160,AT4G22190, AT4G22210, AT4G22214, AT4G22217, AT4G22235, AT4G22270,AT4G22320, AT4G22400, AT4G22420, AT4G22430, AT4G22440, AT4G22470,AT4G22510, AT4G22600, AT4G22740, AT4G22800, AT4G22830, AT4G22850,AT4G22860, AT4G22920, AT4G22980, AT4G23020, AT4G23090, AT4G23110,AT4G23330, AT4G23493, AT4G23496, AT4G23610, AT4G23760, AT4G23770,AT4G23780, AT4G23870, AT4G23880, AT4G23885, AT4G23890, AT4G23910,AT4G23970, AT4G23980, AT4G24030, AT4G24110, AT4G24150, AT4G24175,AT4G24265, AT4G24275, AT4G24300, AT4G24370, AT4G24410, AT4G24500,AT4G24590, AT4G24600, AT4G24610, AT4G24680, AT4G24700, AT4G24720,AT4G24900, AT4G24930, AT4G24950, AT4G24972, AT4G24980, AT4G25030,AT4G25070, AT4G25170, AT4G25315, AT4G25430, AT4G25510, AT4G25515,AT4G25520, AT4G25620, AT4G25670, AT4G25690, AT4G25760, AT4G25845,AT4G26020, AT4G26030, AT4G26040, AT4G26060, AT4G26130, AT4G26170,AT4G26190, AT4G26240, AT4G26290, AT4G26320, AT4G26410, AT4G26450,AT4G26630, AT4G26850, AT4G26960, AT4G26965, AT4G27020, AT4G27030,AT4G27120, AT4G27330, AT4G27380, AT4G27390, AT4G27410, AT4G27415,AT4G27430, AT4G27500, AT4G27510, AT4G27530, AT4G27580, AT4G27595,AT4G27610, AT4G27630, AT4G27652, AT4G27654, AT4G27657, AT4G27660,AT4G27810, AT4G27850, AT4G27930, AT4G27980, AT4G28005, AT4G28025,AT4G28085, AT4G28100, AT4G28160, AT4G28170, AT4G28180, AT4G28190,AT4G28210, AT4G28230, AT4G28240, AT4G28260, AT4G28280, AT4G28290,AT4G28310, AT4G28330, AT4G28340, AT4G28460, AT4G28690, AT4G28740,AT4G28760, AT4G28770, AT4G28810, AT4G28840, AT4G28870, AT4G28920,AT4G28930, AT4G29020, AT4G29030, AT4G29200, AT4G29273, AT4G29280,AT4G29285, AT4G29290, AT4G29300, AT4G29305, AT4G29520, AT4G29560,AT4G29660, AT4G29780, AT4G29790, AT4G29870, AT4G29960, AT4G30010,AT4G30050, AT4G30064, AT4G30070, AT4G30074, AT4G30090, AT4G30130,AT4G30150, AT4G30230, AT4G30390, AT4G30450, AT4G30460, AT4G30630,AT4G30662, AT4G30670, AT4G30730, AT4G30740, AT4G30750, AT4G30770,AT4G30780, AT4G30790, AT4G30970, AT4G30993, AT4G31030, AT4G31080,AT4G31090, AT4G31115, AT4G31260, AT4G31280, AT4G31340, AT4G31380,AT4G31430, AT4G31440, AT4G31510, AT4G31550, AT4G31560, AT4G31570,AT4G31600, AT4G31730, AT4G31805, AT4G31830, AT4G31875, AT4G31880,AT4G31960, AT4G32020, AT4G32030, AT4G32080, AT4G32090, AT4G32100,AT4G32105, AT4G32110, AT4G32190, AT4G32230, AT4G32240, AT4G32460,AT4G32560, AT4G32590, AT4G32680, AT4G32695, AT4G32770, AT4G32860,AT4G32960, AT4G32970, AT4G33130, AT4G33310, AT4G33320, AT4G33380,AT4G33480, AT4G33560, AT4G33590, AT4G33600, AT4G33610, AT4G33625,AT4G33640, AT4G33660, AT4G33666, AT4G33690, AT4G33740, AT4G33750,AT4G33780, AT4G33800, AT4G33890, AT4G33925, AT4G33960, AT4G33970,AT4G33980, AT4G34080, AT4G34090, AT4G34190, AT4G34265, AT4G34412,AT4G34550, AT4G34560, AT4G34600, AT4G34630, AT4G34690, AT4G35170,AT4G35240, AT4G35295, AT4G35360, AT4G35400, AT4G35430, AT4G35510,AT4G35530, AT4G35725, AT4G35940, AT4G35980, AT4G35987, AT4G36100,AT4G36105, AT4G36170, AT4G36370, AT4G36440, AT4G36460, AT4G36500,AT4G36510, AT4G36515, AT4G36520, AT4G36560, AT4G36830, AT4G36925,AT4G36980, AT4G37030, AT4G37090, AT4G37110, AT4G37130, AT4G37240,AT4G37290, AT4G37295, AT4G37300, AT4G37440, AT4G37445, AT4G37450,AT4G37685, AT4G37700, AT4G37740, AT4G37810, AT4G37820, AT4G37920,AT4G37925, AT4G38060, AT4G38070, AT4G38080, AT4G38100, AT4G38120,AT4G38225, AT4G38280, AT4G38330, AT4G38490, AT4G38500, AT4G38680,AT4G38750, AT4G38760, AT4G38820, AT4G38980, AT4G39190, AT4G39235,AT4G39300, AT4G39320, AT4G39360, AT4G39380, AT4G39403, AT4G39420,AT4G39430, AT4G39440, AT4G39450, AT4G39610, AT4G39630, AT4G39670,AT4G39675, AT4G39690, AT4G39745, AT4G39750, AT4G39790, AT4G39840,AT4G39860, AT4G39900, AT4G39917, AT4G39920, AT4G39930, AT4G40020,AT4G40045, AT4G40090, AT5G01015, AT5G01030, AT5G01075, AT5G01080,AT5G01225, AT5G01280, AT5G01350, AT5G01370, AT5G01570, AT5G01690,AT5G01730, AT5G01790, AT5G01840, AT5G01881, AT5G01910, AT5G01970,AT5G02000, AT5G02020, AT5G02090, AT5G02120, AT5G02160, AT5G02200,AT5G02220, AT5G02390, AT5G02420, AT5G02440, AT5G02502, AT5G02510,AT5G02520, AT5G02550, AT5G02640, AT5G02650, AT5G02660, AT5G02680,AT5G02690, AT5G02720, AT5G02740, AT5G02770, AT5G02850, AT5G03050,AT5G03060, AT5G03090, AT5G03110, AT5G03120, AT5G03130, AT5G03210,AT5G03345, AT5G03400, AT5G03420, AT5G03440, AT5G03460, AT5G03545,AT5G03550, AT5G03560, AT5G03670, AT5G03710, AT5G03740, AT5G03750,AT5G03830, AT5G03890, AT5G03900, AT5G03920, AT5G03930, AT5G03950,AT5G03990, AT5G04000, AT5G04030, AT5G04045, AT5G04080, AT5G04090,AT5G04190, AT5G04290, AT5G04470, AT5G04520, AT5G04650, AT5G04670,AT5G04730, AT5G04750, AT5G04790, AT5G04840, AT5G04860, AT5G04910,AT5G05020, AT5G05180, AT5G05190, AT5G05220, AT5G05240, AT5G05250,AT5G05300, AT5G05310, AT5G05360, AT5G05480, AT5G05640, AT5G05660,AT5G05800, AT5G05840, AT5G05930, AT5G05950, AT5G05965, AT5G06010,AT5G06043, AT5G06190, AT5G06240, AT5G06265, AT5G06270, AT5G06280,AT5G06380, AT5G06590, AT5G06610, AT5G06710, AT5G06790, AT5G06890,AT5G06930, AT5G06980, AT5G06990, AT5G07020, AT5G07170, AT5G07330,AT5G07380, AT5G07490, AT5G07570, AT5G07730, AT5G07790, AT5G07890,AT5G07940, AT5G07950, AT5G07970, AT5G07980, AT5G08010, AT5G08040,AT5G08055, AT5G08060, AT5G08090, AT5G08120, AT5G08150, AT5G08185,AT5G08210, AT5G08220, AT5G08240, AT5G08270, AT5G08315, AT5G08320,AT5G08400, AT5G08450, AT5G08505, AT5G08540, AT5G08770, AT5G09225,AT5G09270, AT5G09310, AT5G09390, AT5G09480, AT5G09520, AT5G09530,AT5G09670, AT5G09860, AT5G09960, AT5G09980, AT5G09990, AT5G09995,AT5G10010, AT5G10040, AT5G10110, AT5G10310, AT5G10320, AT5G10430,AT5G10590, AT5G10660, AT5G10670, AT5G10680, AT5G10700, AT5G10710,AT5G10745, AT5G10850, AT5G10890, AT5G10950, AT5G11030, AT5G11070,AT5G11090, AT5G11120, AT5G11220, AT5G11280, AT5G11390, AT5G11420,AT5G11530, AT5G11600, AT5G11630, AT5G11680, AT5G11760, AT5G11780,AT5G11810, AT5G11830, AT5G11870, AT5G11970, AT5G11990, AT5G12010,AT5G12050, AT5G12160, AT5G12170, AT5G12230, AT5G12235, AT5G12240,AT5G12290, AT5G12340, AT5G12360, AT5G12430, AT5G12470, AT5G12880,AT5G12930, AT5G12990, AT5G13090, AT5G13240, AT5G13250, AT5G13260,AT5G13310, AT5G13340, AT5G13390, AT5G13470, AT5G13500, AT5G13540,AT5G13560, AT5G13590, AT5G13610, AT5G13620, AT5G13655, AT5G13660,AT5G13825, AT5G13880, AT5G13940, AT5G13950, AT5G13970, AT5G14020,AT5G14090, AT5G14105, AT5G14110, AT5G14150, AT5G14210, AT5G14290,AT5G14330, AT5G14380, AT5G14410, AT5G14560, AT5G14690, AT5G14710,AT5G14730, AT5G14920, AT5G14930, AT5G14970, AT5G14990, AT5G15000,AT5G15190, AT5G15260, AT5G15320, AT5G15360, AT5G15420, AT5G15560,AT5G15600, AT5G15725, AT5G15780, AT5G15802, AT5G15960, AT5G15970,AT5G15990, AT5G16030, AT5G16060, AT5G16100, AT5G16110, AT5G16160,AT5G16200, AT5G16250, AT5G16453, AT5G16470, AT5G16486, AT5G16520,AT5G16550, AT5G16610, AT5G16660, AT5G16730, AT5G16940, AT5G16950,AT5G17070, AT5G17130, AT5G17160, AT5G17190, AT5G17280, AT5G17340,AT5G17360, AT5G17460, AT5G17510, AT5G17520, AT5G17590, AT5G17610,AT5G17650, AT5G17870, AT5G17910, AT5G18130, AT5G18150, AT5G18250,AT5G18310, AT5G18403, AT5G18407, AT5G18420, AT5G18440, AT5G18540,AT5G18636, AT5G18690, AT5G18710, AT5G18720, AT5G18730, AT5G18740,AT5G18850, AT5G18880, AT5G19070, AT5G19172, AT5G19190, AT5G19230,AT5G19260, AT5G19315, AT5G19340, AT5G19480, AT5G19540, AT5G19570,AT5G19710, AT5G19800, AT5G19810, AT5G19875, AT5G19900, AT5G19950,AT5G19970, AT5G19980, AT5G20045, AT5G20100, AT5G20120, AT5G20130,AT5G20170, AT5G20200, AT5G20370, AT5G20450, AT5G20460, AT5G20610,AT5G20635, AT5G20760, AT5G20770, AT5G20790, AT5G20820, AT5G20935,AT5G21020, AT5G21050, AT5G21070, AT5G21080, AT5G21110, AT5G21125,AT5G21280, AT5G21910, AT5G21940, AT5G22040, AT5G22090, AT5G22120,AT5G22150, AT5G22160, AT5G22170, AT5G22180, AT5G22190, AT5G22210,AT5G22270, AT5G22280, AT5G22310, AT5G22340, AT5G22390, AT5G22430,AT5G22450, AT5G22520, AT5G22530, AT5G22545, AT5G22555, AT5G22650,AT5G22680, AT5G22790, AT5G22875, AT5G22970, AT5G23035, AT5G23100,AT5G23110, AT5G23160, AT5G23180, AT5G23200, AT5G23212, AT5G23390,AT5G23410, AT5G23460, AT5G23490, AT5G23510, AT5G23610, AT5G23640,AT5G23700, AT5G23890, AT5G23920, AT5G24060, AT5G24130, AT5G24165,AT5G24250, AT5G24310, AT5G24313, AT5G24314, AT5G24316, AT5G24355,AT5G24450, AT5G24480, AT5G24500, AT5G24570, AT5G24590, AT5G24610,AT5G24630, AT5G24640, AT5G24655, AT5G24690, AT5G24740, AT5G24860,AT5G24880, AT5G24890, AT5G24920, AT5G24980, AT5G25000, AT5G25070,AT5G25200, AT5G25210, AT5G25240, AT5G25250, AT5G25260, AT5G25265,AT5G25270, AT5G25280, AT5G25360, AT5G25425, AT5G25460, AT5G25500,AT5G25570, AT5G25580, AT5G25590, AT5G25600, AT5G25750, AT5G25754,AT5G25757, AT5G25870, AT5G25920, AT5G26020, AT5G26070, AT5G26080,AT5G26100, AT5G26160, AT5G26230, AT5G26270, AT5G26350, AT5G26620,AT5G26622, AT5G26640, AT5G26650, AT5G26692, AT5G26717, AT5G26720,AT5G26731, AT5G26760, AT5G26770, AT5G26790, AT5G26800, AT5G26805,AT5G26820, AT5G26840, AT5G26850, AT5G26890, AT5G26970, AT5G27020,AT5G27170, AT5G27180, AT5G27210, AT5G27260, AT5G27290, AT5G27330,AT5G27340, AT5G27440, AT5G27500, AT5G27590, AT5G27606, AT5G27710,AT5G27730, AT5G27760, AT5G27800, AT5G27860, AT5G27890, AT5G27940,AT5G27990, AT5G28070, AT5G28090, AT5G28110, AT5G28120, AT5G28130,AT5G28140, AT5G28170, AT5G28190, AT5G28240, AT5G28270, AT5G28295,AT5G28320, AT5G28400, AT5G28410, AT5G28420, AT5G28430, AT5G28440,AT5G28463, AT5G28480, AT5G28482, AT5G28484, AT5G28490, AT5G28500,AT5G28550, AT5G28560, AT5G28600, AT5G28610, AT5G28615, AT5G28620,AT5G28623, AT5G28630, AT5G28720, AT5G28730, AT5G28785, AT5G28790,AT5G28800, AT5G28810, AT5G28820, AT5G28823, AT5G28860, AT5G28910,AT5G28920, AT5G28950, AT5G28960, AT5G28990, AT5G29020, AT5G29050,AT5G29070, AT5G29090, AT5G29210, AT5G29576, AT5G29602, AT5G29613,AT5G30341, AT5G30495, AT5G30520, AT5G31685, AT5G31752, AT5G31753,AT5G31787, AT5G31838, AT5G31873, AT5G32161, AT5G32169, AT5G32312,AT5G32405, AT5G32410, AT5G32590, AT5G32600, AT5G32605, AT5G32610,AT5G32619, AT5G32621, AT5G32775, AT5G33230, AT5G33300, AT5G33303,AT5G33355, AT5G33380, AT5G33390, AT5G33393, AT5G33715, AT5G33806,AT5G33898, AT5G34358, AT5G34450, AT5G34540, AT5G34581, AT5G34820,AT5G34830, AT5G34838, AT5G34860, AT5G34881, AT5G34883, AT5G34887,AT5G34905, AT5G34910, AT5G34970, AT5G34980, AT5G35010, AT5G35020,AT5G35035, AT5G35080, AT5G35090, AT5G35110, AT5G35120, AT5G35195,AT5G35230, AT5G35270, AT5G35290, AT5G35300, AT5G35320, AT5G35460,AT5G35470, AT5G35475, AT5G35480, AT5G35490, AT5G35510, AT5G35540,AT5G35603, AT5G35604, AT5G35646, AT5G35695, AT5G35732, AT5G35737,AT5G35753, AT5G35760, AT5G35792, AT5G35870, AT5G35880, AT5G35890,AT5G35945, AT5G36001, AT5G36020, AT5G36035, AT5G36040, AT5G36060,AT5G36070, AT5G36080, AT5G36100, AT5G36190, AT5G36228, AT5G36280,AT5G36650, AT5G36690, AT5G36710, AT5G36720, AT5G36735, AT5G36780,AT5G36800, AT5G36810, AT5G36900, AT5G36920, AT5G36925, AT5G36960,AT5G37010, AT5G37050, AT5G37120, AT5G37190, AT5G37240, AT5G37360,AT5G37385, AT5G37473, AT5G37474, AT5G37475, AT5G37478, AT5G37480,AT5G37550, AT5G37730, AT5G37840, AT5G37880, AT5G38050, AT5G38060,AT5G38080, AT5G38090, AT5G38190, AT5G38300, AT5G38310, AT5G38317,AT5G38320, AT5G38330, AT5G38380, AT5G38400, AT5G38595, AT5G38660,AT5G38720, AT5G38750, AT5G38770, AT5G38790, AT5G38880, AT5G38980,AT5G39140, AT5G39170, AT5G39200, AT5G39205, AT5G39210, AT5G39240,AT5G39365, AT5G39520, AT5G39530, AT5G39570, AT5G39600, AT5G39790,AT5G39800, AT5G39880, AT5G40070, AT5G40080, AT5G40110, AT5G40155,AT5G40180, AT5G40450, AT5G40460, AT5G40500, AT5G40520, AT5G40595,AT5G40600, AT5G40620, AT5G40640, AT5G40690, AT5G40700, AT5G40710,AT5G40730, AT5G40740, AT5G40800, AT5G40855, AT5G40900, AT5G40960,AT5G40970, AT5G40980, AT5G41020, AT5G41100, AT5G41110, AT5G41140,AT5G41190, AT5G41270, AT5G41320, AT5G41505, AT5G41560, AT5G41620,AT5G41640, AT5G41660, AT5G41780, AT5G41790, AT5G41810, AT5G41860,AT5G41910, AT5G41960, AT5G41980, AT5G42030, AT5G42060, AT5G42070,AT5G42110, AT5G42146, AT5G42223, AT5G42232, AT5G42235, AT5G42242,AT5G42290, AT5G42330, AT5G42530, AT5G42635, AT5G42660, AT5G42710,AT5G42750, AT5G42765, AT5G42780, AT5G42785, AT5G42797, AT5G42825,AT5G42900, AT5G42920, AT5G42955, AT5G42957, AT5G42960, AT5G43000,AT5G43070, AT5G43150, AT5G43185, AT5G43230, AT5G43260, AT5G43285,AT5G43310, AT5G43401, AT5G43405, AT5G43480, AT5G43490, AT5G43510,AT5G43513, AT5G43720, AT5G43750, AT5G43770, AT5G43880, AT5G43970,AT5G44005, AT5G44010, AT5G44040, AT5G44060, AT5G44150, AT5G44313,AT5G44350, AT5G44565, AT5G44570, AT5G44575, AT5G44580, AT5G44650,AT5G44660, AT5G44780, AT5G44820, AT5G44860, AT5G44880, AT5G44973,AT5G45113, AT5G45330, AT5G45350, AT5G45410, AT5G45460, AT5G45573,AT5G45660, AT5G45710, AT5G45740, AT5G45830, AT5G46020, AT5G46115,AT5G46120, AT5G46220, AT5G46295, AT5G46300, AT5G46310, AT5G46320,AT5G46440, AT5G46500, AT5G46620, AT5G46730, AT5G46770, AT5G46795,AT5G46871, AT5G46873, AT5G46874, AT5G46877, AT5G47090, AT5G47110,AT5G47170, AT5G47400, AT5G47410, AT5G47455, AT5G47480, AT5G47490,AT5G47570, AT5G47580, AT5G47635, AT5G47830, AT5G47870, AT5G47920,AT5G47940, AT5G48080, AT5G48175, AT5G48200, AT5G48205, AT5G48210,AT5G48280, AT5G48310, AT5G48335, AT5G48340, AT5G48420, AT5G48470,AT5G48500, AT5G48515, AT5G48520, AT5G48530, AT5G48595, AT5G48605,AT5G48610, AT5G48657, AT5G48720, AT5G48790, AT5G48830, AT5G48860,AT5G48920, AT5G48953, AT5G49015, AT5G49090, AT5G49100, AT5G49110,AT5G49170, AT5G49210, AT5G49250, AT5G49260, AT5G49280, AT5G49380,AT5G49400, AT5G49410, AT5G49440, AT5G49525, AT5G49550, AT5G49590,AT5G49640, AT5G49670, AT5G49680, AT5G49710, AT5G49790, AT5G49830,AT5G49900, AT5G50090, AT5G50175, AT5G50190, AT5G50200, AT5G50290,AT5G50335, AT5G50350, AT5G50360, AT5G50375, AT5G50410, AT5G50420,AT5G50500, AT5G50540, AT5G50560, AT5G50565, AT5G50610, AT5G50645,AT5G50660, AT5G50665, AT5G50710, AT5G50830, AT5G50840, AT5G50880,AT5G50910, AT5G51090, AT5G51105, AT5G51170, AT5G51195, AT5G51200,AT5G51230, AT5G51330, AT5G51390, AT5G51420, AT5G51430, AT5G51510,AT5G51530, AT5G51545, AT5G51580, AT5G51650, AT5G51680, AT5G51840,AT5G51845, AT5G51850, AT5G51960, AT5G52065, AT5G52080, AT5G52110,AT5G52130, AT5G52140, AT5G52200, AT5G52220, AT5G52280, AT5G52290,AT5G52310, AT5G52410, AT5G52420, AT5G52430, AT5G52500, AT5G52530,AT5G52547, AT5G52550, AT5G52580, AT5G52605, AT5G52780, AT5G52800,AT5G52870, AT5G52890, AT5G52900, AT5G52950, AT5G52960, AT5G52965,AT5G52970, AT5G52975, AT5G52980, AT5G53020, AT5G53030, AT5G53045,AT5G53110, AT5G53220, AT5G53280, AT5G53410, AT5G53440, AT5G53620,AT5G53650, AT5G53660, AT5G53670, AT5G53690, AT5G53710, AT5G53740,AT5G53800, AT5G53860, AT5G53880, AT5G53895, AT5G53900, AT5G53905,AT5G53930, AT5G53960, AT5G54062, AT5G54067, AT5G54095, AT5G54145,AT5G54150, AT5G54215, AT5G54350, AT5G54410, AT5G54440, AT5G54460,AT5G54480, AT5G54540, AT5G54585, AT5G54790, AT5G54850, AT5G54870,AT5G54930, AT5G54950, AT5G54970, AT5G55010, AT5G55030, AT5G55060,AT5G55120, AT5G55131, AT5G55132, AT5G55135, AT5G55210, AT5G55330,AT5G55340, AT5G55350, AT5G55360, AT5G55370, AT5G55420, AT5G55430,AT5G55490, AT5G55500, AT5G55507, AT5G55610, AT5G55620, AT5G55640,AT5G55650, AT5G55660, AT5G55680, AT5G55710, AT5G55750, AT5G55790,AT5G55820, AT5G55870, AT5G55893, AT5G55980, AT5G56070, AT5G56075,AT5G56100, AT5G56120, AT5G56170, AT5G56210, AT5G56240, AT5G56250,AT5G56368, AT5G56369, AT5G56520, AT5G56540, AT5G56550, AT5G56770,AT5G56780, AT5G56795, AT5G56850, AT5G56880, AT5G57000, AT5G57060,AT5G57070, AT5G57080, AT5G57180, AT5G57310, AT5G57340, AT5G57345,AT5G57370, AT5G57400, AT5G57410, AT5G57420, AT5G57460, AT5G57500,AT5G57510, AT5G57650, AT5G57685, AT5G57730, AT5G57760, AT5G57790,AT5G57880, AT5G57887, AT5G57910, AT5G58100, AT5G58110, AT5G58210,AT5G58250, AT5G58260, AT5G58360, AT5G58375, AT5G58470, AT5G58500,AT5G58510, AT5G58570, AT5G58630, AT5G58650, AT5G58790, AT5G58880,AT5G58920, AT5G58960, AT5G59050, AT5G59060, AT5G59080, AT5G59105,AT5G59110, AT5G59170, AT5G59210, AT5G59305, AT5G59330, AT5G59350,AT5G59360, AT5G59460, AT5G59500, AT5G59613, AT5G59616, AT5G59830,AT5G59960, AT5G60000, AT5G60030, AT5G60150, AT5G60210, AT5G60240,AT5G60260, AT5G60290, AT5G60330, AT5G60350, AT5G60400, AT5G60430,AT5G60553, AT5G60630, AT5G60650, AT5G60805, AT5G60810, AT5G60840,AT5G60880, AT5G61040, AT5G61090, AT5G61110, AT5G61120, AT5G61200,AT5G61300, AT5G61340, AT5G61360, AT5G61490, AT5G61605, AT5G61630,AT5G61660, AT5G61710, AT5G61865, AT5G61920, AT5G61940, AT5G61950,AT5G61970, AT5G62090, AT5G62140, AT5G62170, AT5G62240, AT5G62270,AT5G62330, AT5G62400, AT5G62440, AT5G62550, AT5G62575, AT5G62623,AT5G62627, AT5G62640, AT5G62750, AT5G62760, AT5G62900, AT5G62960,AT5G63000, AT5G63040, AT5G63050, AT5G63135, AT5G63320, AT5G63340,AT5G63350, AT5G63500, AT5G63540, AT5G63550, AT5G63720, AT5G63740,AT5G63820, AT5G63830, AT5G63905, AT5G64010, AT5G64090, AT5G64130,AT5G64160, AT5G64170, AT5G64180, AT5G64190, AT5G64230, AT5G64310,AT5G64400, AT5G64480, AT5G64510, AT5G64520, AT5G64540, AT5G64550,AT5G64680, AT5G64690, AT5G64770, AT5G64780, AT5G64800, AT5G64816,AT5G64820, AT5G64850, AT5G64870, AT5G64880, AT5G64890, AT5G64900,AT5G64905, AT5G64930, AT5G65120, AT5G65180, AT5G65207, AT5G65250,AT5G65300, AT5G65340, AT5G65390, AT5G65480, AT5G65495, AT5G65540,AT5G65580, AT5G65610, AT5G65660, AT5G65770, AT5G65810, AT5G65880,AT5G65925, AT5G66000, AT5G66030, AT5G66052, AT5G66230, AT5G66250,AT5G66290, AT5G66340, AT5G66440, AT5G66480, AT5G66490, AT5G66580,AT5G66640, AT5G66658, AT5G66740, AT5G66780, AT5G66800, AT5G66815,AT5G66820, AT5G66985, AT5G67020, AT5G67245, AT5G67350, AT5G67390,AT5G67410, AT5G67550, AT5G67600, AT5G67610, AT5G67620, AT5G67640,ATCG00870, ATCG01270, ATMG00010, ATMG00030, ATMG00050, ATMG00120,ATMG00130, ATMG00150, ATMG00170, ATMG00180, ATMG00200, ATMG00260,ATMG00300, ATMG00310, ATMG00320, ATMG00400, ATMG00430, ATMG00440,ATMG00450, ATMG00470, ATMG00500, ATMG00530, ATMG00540, ATMG00550,ATMG00600, ATMG00610, ATMG00620, ATMG00630, ATMG00660, ATMG00670,ATMG00680, ATMG00720, ATMG00740, ATMG00750, ATMG00760, ATMG00770,ATMG00840, ATMG00870, ATMG00880, ATMG00890, ATMG00910, ATMG00920,ATMG00970, ATMG01000, ATMG01010, ATMG01020, ATMG01030, ATMG01040,ATMG01050, ATMG01060, ATMG01090, ATMG01100, ATMG01130, ATMG01140,ATMG01150, ATMG01180, ATMG01200, ATMG01210, ATMG01220, ATMG01230,ATMG01240, ATMG01260, ATMG01290, ATMG01300, ATMG01310, ATMG01330,ATMG01350, ATMG01370, and ATMG01400. With regard to the POFs identifiedin Arabidopsis, the following POFs were identified as specific either toa single species or a single genus (referred to herein as ssPOFs): LocusID AT1G01400, AT1G01810, AT1G01990, AT1G02320, AT1G02350, AT1G02405,AT1G02450, AT1G02490, AT1G02540, AT1G02710, AT1G02965, AT1G03106,AT1G03200, AT1G03240, AT1G03320, AT1G03420, AT1G03660, AT1G04660,AT1G04670, AT1G04800, AT1G05040, AT1G05065, AT1G05085, AT1G05330,AT1G05340, AT1G05450, AT1G05490, AT1G05575, AT1G06135, AT1G06320,AT1G06420, AT1G06475, AT1G06930, AT1G07135, AT1G07190, AT1G07473,AT1G07500, AT1G07680, AT1G07690, AT1G08035, AT1G09415, AT1G10100,AT1G10420, AT1G10990, AT1G11470, AT1G11850, AT1G12080, AT1G12660,AT1G12805, AT1G12810, AT1G12845, AT1G13605, AT1G13607, AT1G13608,AT1G13609, AT1G13620, AT1G13670, AT1G13755, AT1G14755, AT1G15385,AT1G15757, AT1G15825, AT1G15830, AT1G15840, AT1G16025, AT1G16515,AT1G16730, AT1G17090, AT1G17285, AT1G17300, AT1G17510, AT1G17780,AT1G17900, AT1G18220, AT1G18510, AT1G19394, AT1G19397, AT1G19500,AT1G19620, AT1G19960, AT1G20070, AT1G20100, AT1G20280, AT1G20690,AT1G21020, AT1G21323, AT1G21395, AT1G21475, AT1G21520, AT1G21940,AT1G21950, AT1G22010, AT1G22120, AT1G22140, AT1G22335, AT1G22590,AT1G22885, AT1G22890, AT1G23050, AT1G23650, AT1G24060, AT1G24145,AT1G24390, AT1G24575, AT1G24822, AT1G24851, AT1G24938, AT1G24996,AT1G25025, AT1G25097, AT1G25112, AT1G25170, AT1G25180, AT1G25425,AT1G26140, AT1G26210, AT1G26290, AT1G26350, AT1G26710, AT1G26720,AT1G27550, AT1G27610, AT1G27640, AT1G27670, AT1G27695, AT1G27710,AT1G27790, AT1G28135, AT1G28375, AT1G28630, AT1G29010, AT1G29179,AT1G29355, AT1G29480, AT1G29530, AT1G29540, AT1G29560, AT1G29580,AT1G29610, AT1G29620, AT1G30250, AT1G30515, AT1G30757, AT1G30795,AT1G30814, AT1G30835, AT1G31060, AT1G31150, AT1G31270, AT1G31335,AT1G31520, AT1G31580, AT1G31620, AT1G31750, AT1G31772, AT1G31835,AT1G31960, AT1G31990, AT1G32000, AT1G32010, AT1G32040, AT1G32290,AT1G32337, AT1G32570, AT1G32630, AT1G32650, AT1G32670, AT1G32680,AT1G32920, AT1G32928, AT1G32975, AT1G33135, AT1G33607, AT1G33640,AT1G33820, AT1G33860, AT1G34047, AT1G34095, AT1G34280, AT1G34315,AT1G34400, AT1G34440, AT1G34590, AT1G34730, AT1G34910, AT1G35030,AT1G35040, AT1G35080, AT1G35100, AT1G35183, AT1G35230, AT1G35320,AT1G35375, AT1G35435, AT1G35500, AT1G35513, AT1G35614, AT1G35617,AT1G35640, AT1G35820, AT1G35880, AT1G35890, AT1G35900, AT1G36100,AT1G36230, AT1G36395, AT1G36640, AT1G36670, AT1G36675, AT1G36745,AT1G36756, AT1G36763, AT1G36925, AT1G36960, AT1G36970, AT1G37000,AT1G37015, AT1G37037, AT1G37045, AT1G38380, AT1G38630, AT1G38790,AT1G38950, AT1G39350, AT1G40080, AT1G40115, AT1G40125, AT1G40129,AT1G40133, AT1G40230, AT1G41650, AT1G41750, AT1G41770, AT1G41810,AT1G41820, AT1G41855, AT1G41870, AT1G41900, AT1G42080, AT1G42190,AT1G42367, AT1G42393, AT1G42515, AT1G42580, AT1G42630, AT1G42700,AT1G42740, AT1G43205, AT1G43230, AT1G43320, AT1G43415, AT1G43720,AT1G43777, AT1G43810, AT1G43920, AT1G43940, AT1G43970, AT1G44085,AT1G44222, AT1G44740, AT1G44850, AT1G44875, AT1G44930, AT1G44990,AT1G45165, AT1G45403, AT1G46336, AT1G47280, AT1G47317, AT1G47395,AT1G47400, AT1G47485, AT1G47495, AT1G47660, AT1G47680, AT1G47690,AT1G47700, AT1G47770, AT1G47813, AT1G48145, AT1G48250, AT1G48325,AT1G48730, AT1G49110, AT1G49150, AT1G49500, AT1G49680, AT1G49715,AT1G49940, AT1G50080, AT1G50220, AT1G50290, AT1G50350, AT1G50530,AT1G50800, AT1G50930, AT1G51000, AT1G51010, AT1G51030, AT1G51430,AT1G51915, AT1G51970, AT1G52087, AT1G52090, AT1G52390, AT1G52410,AT1G52550, AT1G52615, AT1G52827, AT1G52840, AT1G52905, AT1G53265,AT1G53285, AT1G53480, AT1G53610, AT1G53620, AT1G53625, AT1G53640,AT1G53785, AT1G53935, AT1G53970, AT1G54420, AT1G54445, AT1G54575,AT1G54640, AT1G54720, AT1G54880, AT1G54923, AT1G54926, AT1G54950,AT1G55220, AT1G55330, AT1G55400, AT1G55675, AT1G55710, AT1G55990,AT1G56085, AT1G56270, AT1G56415, AT1G56530, AT1G56553, AT1G56555,AT1G56660, AT1G58055, AT1G58150, AT1G58225, AT1G58235, AT1G58242,AT1G59535, AT1G59722, AT1G59865, AT1G59885, AT1G60240, AT1G60983,AT1G60987, AT1G61090, AT1G61095, AT1G61097, AT1G61200, AT1G61688,AT1G61920, AT1G62000, AT1G62060, AT1G62070, AT1G62080, AT1G62210,AT1G62220, AT1G62225, AT1G62240, AT1G62480, AT1G62690, AT1G62935,AT1G63055, AT1G63105, AT1G63240, AT1G63522, AT1G63535, AT1G63960,AT1G64107, AT1G64360, AT1G64370, AT1G64405, AT1G64490, AT1G64560,AT1G64800, AT1G65342, AT1G65352, AT1G65490, AT1G65500, AT1G65510,AT1G65845, AT1G66145, AT1G66245, AT1G66790, AT1G66820, AT1G67350,AT1G67670, AT1G67775, AT1G67855, AT1G67860, AT1G67865, AT1G67870,AT1G68250, AT1G68430, AT1G68725, AT1G68845, AT1G68870, AT1G68875,AT1G68905, AT1G68907, AT1G68935, AT1G68945, AT1G69050, AT1G69470,AT1G69760, AT1G69825, AT1G69970, AT1G70350, AT1G70470, AT1G70895,AT1G70990, AT1G71235, AT1G71470, AT1G71910, AT1G72080, AT1G72580,AT1G72600, AT1G72645, AT1G73130, AT1G73177, AT1G73510, AT1G73603,AT1G73607, AT1G74045, AT1G74055, AT1G75190, AT1G75770, AT1G75870,AT1G76230, AT1G76820, AT1G76840, AT1G76910, AT1G76955, AT1G76960,AT1G76965, AT1G77655, AT1G77765, AT1G77885, AT1G77910, AT1G77960,AT1G78030, AT1G79170, AT1G80610, AT1G80865, AT2G01031, AT2G01175,AT2G01310, AT2G01400, AT2G02280, AT2G02440, AT2G02490, AT2G02515,AT2G02795, AT2G02835, AT2G02840, AT2G03180, AT2G03310, AT2G03320,AT2G03540, AT2G03570, AT2G03580, AT2G03830, AT2G03932, AT2G03937,AT2G04000, AT2G04025, AT2G04034, AT2G04045, AT2G04046, AT2G04063,AT2G04320, AT2G04370, AT2G04380, AT2G04410, AT2G04515, AT2G04600,AT2G04675, AT2G04800, AT2G04870, AT2G04925, AT2G05000, AT2G05117,AT2G05185, AT2G05270, AT2G05350, AT2G05500, AT2G05564, AT2G05645,AT2G05647, AT2G05752, AT2G05915, AT2G06095, AT2G06140, AT2G06166,AT2G06230, AT2G06390, AT2G06420, AT2G06480, AT2G06555, AT2G06570,AT2G06620, AT2G06630, AT2G06645, AT2G06750, AT2G06775, AT2G06906,AT2G06908, AT2G06914, AT2G07000, AT2G07190, AT2G07215, AT2G07280,AT2G07290, AT2G07310, AT2G07505, AT2G07669, AT2G07672, AT2G07673,AT2G07674, AT2G07676, AT2G07678, AT2G07691, AT2G07692, AT2G07701,AT2G07702, 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AT2G14460, AT2G14590, AT2G14600,AT2G14700, AT2G14774, AT2G14800, AT2G14810, AT2G14890, AT2G14935,AT2G15185, AT2G15327, AT2G15340, AT2G15345, AT2G15420, AT2G15535,AT2G15550, AT2G15600, AT2G15800, AT2G15815, AT2G15830, AT2G15930,AT2G15960, AT2G16015, AT2G16020, AT2G16170, AT2G16340, AT2G16410,AT2G16575, AT2G16586, AT2G16676, AT2G16820, AT2G17442, AT2G17540,AT2G17723, AT2G17960, AT2G18070, AT2G18200, AT2G18270, AT2G18440,AT2G18610, AT2G18920, AT2G18930, AT2G18970, AT2G19200, AT2G19290,AT2G19300, AT2G19320, AT2G19420, AT2G19700, AT2G19802, AT2G19850,AT2G19893, AT2G20150, AT2G20208, AT2G20250, AT2G20463, AT2G20595,AT2G20620, AT2G20625, AT2G20870, AT2G20970, AT2G21185, AT2G21237,AT2G21465, AT2G21725, AT2G21780, AT2G22000, AT2G22080, AT2G22121,AT2G22122, AT2G22320, AT2G22340, AT2G22470, AT2G22510, AT2G22520,AT2G22805, AT2G22807, AT2G22820, AT2G22905, AT2G22940, AT2G22941,AT2G23040, AT2G23130, AT2G23440, AT2G23490, AT2G23920, AT2G23985,AT2G24285, AT2G24310, AT2G24340, AT2G24460, AT2G24617, AT2G24625,AT2G24780, AT2G24910, AT2G24945, AT2G25185, AT2G25250, AT2G25510,AT2G25565, AT2G25685, AT2G25990, AT2G26120, AT2G26880, AT2G27250,AT2G27315, AT2G27380, AT2G27390, AT2G27535, AT2G27540, AT2G28330,AT2G28570, AT2G28625, AT2G29045, AT2G29180, AT2G29790, AT2G29920,AT2G29995, AT2G30430, AT2G30560, AT2G30760, AT2G30925, AT2G30930,AT2G30960, AT2G30985, AT2G31035, AT2G31345, AT2G31590, AT2G31700,AT2G31751, AT2G31850, AT2G32275, AT2G32890, AT2G33233, AT2G34010,AT2G34100, AT2G34110, AT2G34120, AT2G34123, AT2G34185, AT2G34220,AT2G34230, AT2G34270, AT2G34310, AT2G34330, AT2G34655, AT2G34800,AT2G34870, AT2G35070, AT2G35080, AT2G35090, AT2G35733, AT2G35750,AT2G35870, AT2G36030, AT2G36040, AT2G36255, AT2G36440, AT2G36695,AT2G36724, AT2G36920, AT2G36940, AT2G37070, AT2G37300, AT2G37910,AT2G38350, AT2G38690, AT2G38823, AT2G39160, AT2G39520, AT2G39680,AT2G40020, AT2G40085, AT2G40530, AT2G40765, AT2G40955, AT2G41230,AT2G41260, AT2G41280, AT2G41390, AT2G41400, AT2G41420, AT2G41440,AT2G41650, 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AT3G15780, AT3G15860, AT3G15910,AT3G16750, AT3G16895, AT3G17155, AT3G17190, AT3G18250, AT3G18485,AT3G18540, AT3G18700, AT3G19030, AT3G19055, AT3G19530, AT3G19790,AT3G20155, AT3G20362, AT3G20555, AT3G20850, AT3G20865, AT3G20900,AT3G21570, AT3G21680, AT3G22070, AT3G22090, AT3G22231, AT3G22235,AT3G22240, AT3G22415, AT3G23040, AT3G23165, AT3G23167, AT3G23172,AT3G23245, AT3G23295, AT3G23715, AT3G23720, AT3G23727, AT3G23850,AT3G24225, AT3G24250, AT3G24280, AT3G24380, AT3G24508, AT3G24510,AT3G24513, AT3G24517, AT3G24640, AT3G24770, AT3G25080, AT3G25200,AT3G25655, AT3G25727, AT3G25882, AT3G25905, AT3G26110, AT3G26235,AT3G26616, AT3G26800, AT3G27025, AT3G27370, AT3G27590, AT3G27800,AT3G27906, AT3G27990, AT3G28110, AT3G28120, AT3G28170, AT3G28190,AT3G28240, AT3G28260, AT3G28280, AT3G28420, AT3G28530, AT3G28590,AT3G29034, AT3G29080, AT3G29140, AT3G29210, AT3G29300, AT3G29305,AT3G29560, AT3G29570, AT3G29600, AT3G29610, AT3G29700, AT3G29786,AT3G29790, AT3G29796, AT3G30150, AT3G30160, AT3G30220, AT3G30250,AT3G30320, AT3G30350, AT3G30360, AT3G30490, AT3G30510, AT3G30520,AT3G30580, AT3G30590, AT3G30610, AT3G30645, AT3G30650, AT3G30660,AT3G30670, AT3G30690, AT3G30700, AT3G30720, AT3G30750, AT3G30751,AT3G30755, AT3G30816, AT3G30820, AT3G30840, AT3G30845, AT3G31300,AT3G31310, AT3G31320, AT3G31330, AT3G31350, AT3G31370, AT3G31400,AT3G31406, AT3G31540, AT3G31910, AT3G31915, AT3G31940, AT3G31955,AT3G32050, AT3G32070, AT3G32100, AT3G32120, AT3G32150, AT3G32160,AT3G32180, AT3G32190, AT3G32200, AT3G32896, AT3G32902, AT3G32903,AT3G32904, AT3G32960, AT3G33064, AT3G33073, AT3G33080, AT3G33131,AT3G33187, AT3G33230, AT3G33293, AT3G33393, AT3G33448, AT3G33494,AT3G33572, AT3G42070, AT3G42090, AT3G42120, AT3G42130, AT3G42140,AT3G42190, AT3G42200, AT3G42240, AT3G42254, AT3G42300, AT3G42310,AT3G42380, AT3G42390, AT3G42430, AT3G42436, AT3G42473, AT3G42480,AT3G42490, AT3G42510, AT3G42520, AT3G42540, AT3G42556, AT3G42590,AT3G42610, AT3G42680, AT3G42700, AT3G42723, AT3G42740, AT3G42750,AT3G42780, 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AT4G06603,AT4G06637, AT4G06672, AT4G06716, AT4G06724, AT4G06728, AT4G06735,AT4G06740, AT4G07380, AT4G07452, AT4G07460, AT4G07485, AT4G07500,AT4G07523, AT4G07526, AT4G07666, AT4G07675, AT4G07740, AT4G07825,AT4G07868, AT4G07932, AT4G07943, AT4G07965, AT4G08028, AT4G08039,AT4G08097, AT4G08098, AT4G08111, AT4G08130, AT4G08270, AT4G08336,AT4G08395, AT4G08485, AT4G08555, AT4G08593, AT4G08602, AT4G08710,AT4G08730, AT4G08740, AT4G08760, AT4G08868, AT4G08869, AT4G08874,AT4G08875, AT4G09030, AT4G09153, AT4G09210, AT4G09220, AT4G09260,AT4G09270, AT4G09290, AT4G09390, AT4G09647, AT4G09840, AT4G09850,AT4G09860, AT4G09880, AT4G09984, AT4G10820, AT4G10845, AT4G10860,AT4G10870, AT4G11020, AT4G11100, AT4G11385, AT4G11393, AT4G11700,AT4G11870, AT4G11940, AT4G12005, AT4G12220, AT4G12380, AT4G12580,AT4G12735, AT4G12930, AT4G12940, AT4G12990, AT4G13095, AT4G13150,AT4G13195, AT4G13235, AT4G13320, AT4G13470, AT4G13955, AT4G13968,AT4G14104, AT4G14120, AT4G14315, AT4G14530, AT4G14650, AT4G14810,AT4G15096, AT4G15150, AT4G15460, AT4G15563, AT4G15650, AT4G15710,AT4G15733, AT4G15735, AT4G15950, AT4G15990, AT4G16000, AT4G16040,AT4G16090, AT4G16140, AT4G16215, AT4G16240, AT4G16447, AT4G16460,AT4G16515, AT4G16840, AT4G16980, AT4G17700, AT4G17713, AT4G17930,AT4G17990, AT4G18000, AT4G18080, AT4G18090, AT4G18280, AT4G18310,AT4G18395, AT4G18420, AT4G18490, AT4G18500, AT4G18501, AT4G18510,AT4G18580, AT4G18823, AT4G18850, AT4G18860, AT4G19095, AT4G19200,AT4G19240, AT4G19270, AT4G19280, AT4G19290, AT4G19305, AT4G19320,AT4G19480, AT4G19620, AT4G19905, AT4G20095 AT4G20250, AT4G20290,AT4G20470, AT4G20500, AT4G20510, AT4G20690, AT4G20715, AT4G21215,AT4G21865, AT4G21920, AT4G21950, AT4G22210, AT4G22214, AT4G22217,AT4G22235, AT4G22420, AT4G22430, AT4G22440, AT4G22510, AT4G22800,AT4G23090, AT4G23110, AT4G23493, AT4G23760, AT4G23770, AT4G23780,AT4G23870, AT4G23970, AT4G24030, AT4G24275, AT4G24300, AT4G24410,AT4G24600, AT4G24950, AT4G25510, AT4G26030, AT4G26040, AT4G26290,AT4G26320, AT4G26960, AT4G27415, AT4G27530, AT4G27580, AT4G27652,AT4G27654, AT4G27657, AT4G27850, AT4G27930, AT4G28085, AT4G28160,AT4G28180, AT4G28460, AT4G28810, AT4G28870, AT4G28920, AT4G28930,AT4G29020, AT4G29030, AT4G29200, AT4G29273, AT4G29280, AT4G29285,AT4G29290, AT4G29300, AT4G29305, AT4G30050, AT4G30064, AT4G30450,AT4G30460, AT4G30662, AT4G30670, AT4G30730, AT4G30750, AT4G30970,AT4G31030, AT4G31260, AT4G31280, AT4G31875, AT4G31960, AT4G32080,AT4G32230, AT4G32240, AT4G33310, AT4G33560, AT4G33610, AT4G33660,AT4G33666, AT4G33750, AT4G33960, AT4G34690, AT4G35170, AT4G35400,AT4G35430, AT4G35725, AT4G36170, AT4G36370, AT4G36460, AT4G36510,AT4G36515, AT4G36560, AT4G36925, AT4G37295, AT4G37450, AT4G37685,AT4G38080, AT4G38330, AT4G38820, AT4G39320, AT4G39360, AT4G39403,AT4G39675, AT4G39745, AT4G39917, AT4G39930, AT4G40090, AT5G01080,AT5G01881, AT5G02000, AT5G02520, AT5G02550, AT5G02650, AT5G02690,AT5G03060, AT5G03090, AT5G03130, AT5G03210, AT5G03400, AT5G03545,AT5G03550, AT5G03710, AT5G03920, AT5G03930, AT5G03950, AT5G04030,AT5G04045, AT5G04470, AT5G04650, AT5G04790, AT5G05020, AT5G05640,AT5G05965, AT5G06043, AT5G06190, AT5G06380, AT5G06980, AT5G07730,AT5G08090, AT5G08150, AT5G08185, AT5G08210, AT5G08220, AT5G08505,AT5G09480, AT5G09520, AT5G09980, AT5G09990, AT5G10040, AT5G10430,AT5G10590, AT5G10670, AT5G10745, AT5G11120, AT5G11830, AT5G11990,AT5G12880, AT5G12990, AT5G13825, AT5G14330, AT5G14380, AT5G14560,AT5G14730, AT5G15000, AT5G15190, AT5G15360, AT5G15420, AT5G15560,AT5G15725, AT5G15960, AT5G15970, AT5G15990, AT5G17130, AT5G17340,AT5G17360, AT5G17590, AT5G17650, AT5G18403, AT5G18407, AT5G18690,AT5G19172, AT5G19800, AT5G19810, AT5G20460, AT5G20760, AT5G20770,AT5G21020, AT5G21110, AT5G21125, AT5G21910, AT5G22150, AT5G22160,AT5G22170, AT5G22180, AT5G22190, AT5G22520, AT5G22530, AT5G22545,AT5G22555, AT5G22680, AT5G22970, AT5G23035, AT5G23180, AT5G23212,AT5G23460, AT5G23640, AT5G24250, AT5G24313, AT5G24316, AT5G24480,AT5G24570, AT5G24590, AT5G25000, AT5G25210, AT5G25425, AT5G25600,AT5G25750, AT5G25870, AT5G25920, AT5G26020, AT5G26070, AT5G26080,AT5G26100, AT5G26270, AT5G26350, AT5G26622, AT5G26692, AT5G26717,AT5G26720, AT5G26800, AT5G26840, AT5G26890, AT5G26970, AT5G27170,AT5G27180, AT5G27340, AT5G27440, AT5G27500, AT5G27590, AT5G27606,AT5G27800, AT5G27890, AT5G28070, AT5G28090, AT5G28110, AT5G28120,AT5G28130, AT5G28140, AT5G28170, AT5G28190, AT5G28240, AT5G28270,AT5G28295, AT5G28410, AT5G28430, AT5G28463, AT5G28480, AT5G28482,AT5G28484, AT5G28560, AT5G28600, AT5G28610, AT5G28620, AT5G28623,AT5G28630, AT5G28720, AT5G28785, AT5G28790, AT5G28800, AT5G28810,AT5G28820, AT5G28860, AT5G28920, AT5G28990, AT5G29020, AT5G29050,AT5G29070, AT5G29090, AT5G29210, AT5G29576, AT5G29602, AT5G29613,AT5G30341, AT5G30520, AT5G31685, AT5G31752, AT5G31753, AT5G31787,AT5G31838, AT5G31873, AT5G32161, AT5G32169, AT5G32312, AT5G32405,AT5G32410, AT5G32590, AT5G32600, AT5G32605, AT5G32610, AT5G32619,AT5G32775, AT5G33230, AT5G33355, AT5G33380, AT5G33390, AT5G33393,AT5G33715, AT5G33806, AT5G33898, AT5G34358, AT5G34450, AT5G34581,AT5G34820, AT5G34830, AT5G34910, AT5G34970, AT5G34980, AT5G35010,AT5G35020, AT5G35035, AT5G35090, AT5G35195, AT5G35230, AT5G35270,AT5G35290, AT5G35300, AT5G35470, AT5G35480, AT5G35490, AT5G35510,AT5G35540, AT5G35603, AT5G35604, AT5G35646, AT5G35737, AT5G35760,AT5G35792, AT5G35880, AT5G35890, AT5G35945, AT5G36020, AT5G36035,AT5G36040, AT5G36060, AT5G36070, AT5G36080, AT5G36190, AT5G36650,AT5G36720, AT5G36735, AT5G36900, AT5G36920, AT5G36925, AT5G36960,AT5G37120, AT5G37240, AT5G37385, AT5G37473, AT5G37474, AT5G37880,AT5G38080, AT5G38090, AT5G38190, AT5G38310, AT5G38400, AT5G38595,AT5G38790, AT5G38980, AT5G39140, AT5G39170, AT5G39205, AT5G39365,AT5G39570, AT5G39880, AT5G40070, AT5G40110, AT5G40155, AT5G40180,AT5G40595, AT5G40620, AT5G40730, AT5G40855, AT5G41320, AT5G41640,AT5G41660, AT5G42110, AT5G42146, AT5G42223, AT5G42232, AT5G42235,AT5G42242, AT5G42530, AT5G42635, AT5G42797, AT5G42825, AT5G43000,AT5G43185, AT5G43285, AT5G43401, AT5G43405, AT5G43480, AT5G43510,AT5G43513, AT5G43770, AT5G44005, AT5G44313, AT5G44565, AT5G44570,AT5G44575, AT5G44580, AT5G44880, AT5G44973, AT5G45573, AT5G46115,AT5G46120, AT5G46300, AT5G46310, AT5G46320, AT5G46500, AT5G46730,AT5G46770, AT5G46871, AT5G46873, AT5G46874, AT5G46877, AT5G47170,AT5G48175, AT5G48200, AT5G48280, AT5G48420, AT5G48515, AT5G48530,AT5G48595, AT5G48605, AT5G48657, AT5G48860, AT5G48953, AT5G49015,AT5G49090, AT5G49250, AT5G49260, AT5G49280, AT5G49440, AT5G49590,AT5G49640, AT5G49790, AT5G50190, AT5G50335, AT5G50500, AT5G50540,AT5G50565, AT5G50610, AT5G50645, AT5G50665, AT5G50710, AT5G50880,AT5G50910, AT5G51090, AT5G51105, AT5G51195, AT5G51390, AT5G51580,AT5G51650, AT5G51845, AT5G52080, AT5G52130, AT5G52547, AT5G52965,AT5G53410, AT5G53740, AT5G53895, AT5G53905, AT5G53960, AT5G54067,AT5G54095, AT5G54145, AT5G54215, AT5G54350, AT5G54410, AT5G54460,AT5G54585, AT5G54790, AT5G54970, AT5G55010, AT5G55131, AT5G55132,AT5G55135, AT5G55420, AT5G55430, AT5G55507, AT5G55650, AT5G55680,AT5G55750, AT5G55790, AT5G55870, AT5G55893, AT5G56100, AT5G56368,AT5G56369, AT5G56795, AT5G56880, AT5G57310, AT5G57400, AT5G57650,AT5G57730, AT5G57760, AT5G57790, AT5G57887, AT5G58570, AT5G58650,AT5G59060, AT5G59105, AT5G59170, AT5G59330, AT5G59360, AT5G60000,AT5G60240, AT5G60260, AT5G60290, AT5G60330, AT5G60350, AT5G60400,AT5G60553, AT5G60650, AT5G60805, AT5G60810, AT5G61110, AT5G61120,AT5G61360, AT5G61605, AT5G61660, AT5G61710, AT5G62330, AT5G62400,AT5G62623, AT5G62627, AT5G62750, AT5G63340, AT5G63720, AT5G63820,AT5G63905, AT5G64540, AT5G64690, AT5G64770, AT5G64800, AT5G64890,AT5G64900, AT5G64905, AT5G65300, AT5G65390, AT5G65495, AT5G65580,AT5G65610, AT5G65880, AT5G65925, AT5G66000, AT5G66052, AT5G66340,AT5G66658, AT5G66985, AT5G67245, AT5G67350, AT5G67600, AT5G67640,ATCG00870, ATCG01270, ATMG00030, ATMG00050, ATMG00120, ATMG00130,ATMG00150, ATMG00200, ATMG00260, ATMG00320, ATMG00400, ATMG00430,ATMG00440, ATMG00450, ATMG00470, ATMG00500, ATMG00530, ATMG00540,ATMG00600, ATMG00610, ATMG00630, ATMG00670, ATMG00680, ATMG00720,ATMG00740, ATMG00760, ATMG00770, ATMG00840, ATMG00870, ATMG00880,ATMG00890, ATMG00920, ATMG00970, ATMG01000, ATMG01010, ATMG01020,ATMG01030, ATMG01040, ATMG01050, ATMG01060, ATMG01090, ATMG01100,ATMG01130, ATMG01140, ATMG01150, ATMG01180, ATMG01200, ATMG01210,ATMG01230, ATMG01240, ATMG01260, ATMG01290, ATMG01300, ATMG01310,ATMG01350, ATMG01370, and ATMG01400. Such POFs can be analyzed (e.g.,knock-out mutation, over-expression; etc.) for their ability to modifythe amount of at least one biochemical component in the plant species inwhich it was found, i.e., Arabidopsis for the POFs identified above.After it has been determined that a POF can modify the amount of atleast one biochemical component in the plant species in which it wasfound, the POF can be introduced (e.g., in accordance with the methodsdescribed herein and known in the art) into another plant species, whichis preferably from another genus and the WT of which does not containthe POF, and analyzed for its ability to modify the amount of at leastone biochemical component in that plant species. When the proteindatabase was searched with a protein query using the NCBI BLAST program,QQS had an Expect value of 4e-29 to itself only as determined using themethod of compositional matrix adjust.

EXAMPLES

The following examples serve to illustrate the present disclosure. Theexamples are not intended to limit the scope of the claimed invention.

Example 1

This example describes the construction of a vector for expression ofQQS in plants other than Arabidopsis.

A 35S::QQS fusion construct was made by cloning the amplified,full-length, coding sequence into a binary vector pB2GW7 as shown inFIG. 3. QQS was expressed under the control of the 35S promoter.35S::QQS-pB2GW7 vector was transformed into Agrobacterium tumefaciensstrain EHA101.

Example 2

This example describes the transformation and selection of transgenic,QQS-expressing soybean lines.

The cultivar Williams 82 was transformed by Agrobacterium-mediatedtransformation using the half-seed explants method (Paz et al.,Euphytica 136: 167-179 (2004)). The transformation and selection of R1plants were performed in the Plant Transformation Facility at Iowa StateUniversity. Soybean plants from independent transformations wereselected based on Bar resistance.

Example 3

This example describes the analysis of starch content of the leaves ofsoybean expressing QQS as compared to WT soybean.

Transgenic, QQS-expressing soybean lines (ST94-32#1, ST94-32#2 andST94-16#1) and a WT soybean line (Williams 82) were grown in pots withthree plants/pot under controlled environmental conditions of 16 hoursof light at 27° C. and 8 hours of dark at 20° C. in a growth chamber.The pots were placed randomly in the growth chamber.

Leaves from the top nodes of three individual plants (500-1,000 mg freshweight) were pooled and boiled in 50 mL 80% (v/v) ethanol. The boiledleaves were then ground with a mortar and pestle in 80% ethanol andcentrifuged for 10 min at 13,000 rpm. The resulting pellet was washedtwice with 80% ethanol. After washing, the insoluble material wassuspended in 10 mL of distilled water and boiled for 30 min. Totalstarch (D-glucose) in each leaf sample was quantified usingamyloglucosidase and Megazyme's GOPOD format (Megazyme InternationalIreland Ltd., Wicklow, Ireland). This analysis was conducted intriplicate. The results are shown in Table 1 and FIG. 2.

Example 4

This example describes the analysis of oil, protein and moisturecontents of the seeds of soybean expressing QQS as compared to WTsoybean.

The oil, protein, and moisture contents of seeds were measured bynuclear magnetic resonance (NMR) with the Minispec-mq-one (BRUKER OpticsInc., The Woodlands, Tex.). Seeds were placed in a 10 mm glass tube andweighed. The tube was placed in the Minispec-mq-one and on-screeninstructions were followed. The software provided with theMinispec-mq-one calculates the % of oil, protein, and moisture asdesired. Data were transformed from fresh weight to dry weight. Theresults are shown in Table 1 and FIG. 2.

Example 5

This example describes the analysis of total RNA of the leaves ofsoybean expressing QQS as compared to WT soybean.

Total RNA was extracted from the pooled leaf samples of Example 1 usingthe TRIzol RNA isolation method. Total RNA was purified using the QIAGENRNeasy Mini Kit (QIAGEN, Valencia, Calif.).

One microgram of total RNA was reverse-transcribed using 200 ng randomhexamers (Invitrogen, Paisley, UK) and Superscript II reversetranscriptase (Invitrogen) according to the supplier's instructions.cDNA was stored at 4° C. until amplified using polymerase chain reaction(PCR).

Specific primers and fluorogenic probes for QQS were purchased fromApplied Biosystems (At03403756_sH UBQ10; Warrington, UK) for use in PCR.The 18S ribosomal RNA (18S rRNA) control Reagent (Applied Biosystems)was used as an endogenous control to normalize for differences in theamount of total RNA in each sample and for RNA quality control (Zhong etal., Biochem. Biophys. Res. Comm. 259: 523-526 (1999); Schmittgen etal., J. Biochem. Biophys. Meth. 46: 69-81 (2000); and Bhatia et al.,Anal. Biochem. 216: 223-226 (1994)). PCR reactions were performed usingthe 7500 Fast Real-Time PCR System (Applied Biosystems). Each reactionwas performed in 25 μl reactions and contained the equivalent of 5 ng ofreverse-transcribed RNA (1 ng RNA for the 18S analyses), 50% TaqMan2×PCR Master Mix (PE Applied Biosystems), and 1.25 μl of primer/probemix as recommended by the manufacturer. Conditions for the PCR reactionwere 10 min at 95° C. and then 40 cycles, each consisting of 15 sec at95° C., and 1 min at 60° C.

The relative RNA levels within the samples were determined by generatingstandard curves for the PCR reaction by using the cDNA from one sampleand making 2-fold serial dilutions covering the range equivalent to 100ng-3.125 ng of total RNA (for 18S rRNA analyses, the range was 4ng-0.125 ng). Samples were analysed using the standard curve methoddescribed by Applied Biosystems. Those samples, which showed more than 2cycle threshold (Ct) variation from the median 18S rRNA Ct value, wereexcluded so that expression profiles were not distorted. The results areshown in Table 1.

TABLE 1 Starch Oil Protein QQS/18S rRNA (mg/g fresh wt; (% of dry wt; (%of dry wt; Fresh Weight Sample (by RT-PCR) % decrease) % decrease) %increase) (mean g per seed) QQS-Williams 82 81.78 4.64*; 70.18% 19.05*;5.00% 51.24**; 60.63% 0.162 #1 QQS-Williams 82 4.57 2.71*; 82.58%16.76**; 16.41% 42.31**; 32.63% 0.161 #2 QQS-Williams 82 15.81 7.51*;51.74% 17.39**; 13.27% 46.92**; 47.08% 0.156 #11 Williams 82 0.00 15.5620.05 31.9 0.159 *Student's t-test, P < 0.05 **Student's t-test, P <0.01

As shown in Table 1 and FIG. 2, the starch content of leaves of soybeanlines expressing QQS is significantly lower than the starch content ofleaves of WT soybean (Williams 82). The oil content of seeds ofQQS-expressing soybean is also lower than the oil content of seeds of WTsoybean. In distinct contrast to the starch content of leaves and theoil content of seeds, however, the protein content of seeds ofQQS-expressing soybean is significantly higher than the protein contentof seeds of WT soybean.

Example 6

This example describes the analysis of oil, protein and moisturecontents of the seeds of soybean expressing QQS as compared to WTsoybean and grown in a growth chamber, a greenhouse, or a field.

Transgenic, QQS-expressing soybean lines (ST94-32#1, ST94-32#2 andST94-16#1) and a WT soybean line (Williams 82) were grown in pots withthree plants/pot under controlled environmental conditions of 16 hoursof light at 27° C. and 8 hours of dark at 20° C. in a growth chamber.The pots were placed randomly in the growth chamber.

Transgenic, QQS-expressing soybean lines (ST94-32#1, ST94-32#2 andST94-16#1) and a WT soybean line (Williams 82) were grown in pots withthree plants/pot under controlled environmental conditions of 16 hoursof light at 27° C. and 8 hours of dark at 20° C. in a greenhouse. Thepots were placed randomly in the greenhouse.

Transgenic, QQS-expressing soybean lines (ST94-32#1, ST94-32#2 andST94-16#1) and a WT soybean line (Williams 82) were grown in a field inAmes, Iowa Five replicates, each containing one row of each line, wereplanted.

The oil, protein, and moisture contents of seeds were measured bynear-infrared spectroscopy (NIRS). Data were adjusted to a moisturecontent of 10.97%. The data are shown in Tables 2-4. As shown in Table2, protein is increased (up to 16.85%) and oil and fiber are decreasedin QQS-expressing mutant soybean plants grown in a growth chamber. Asshown in Table 3, protein is increased (up to 13.5%), and oil, fiber andcarbohydrates are decreased in QQS-expressing mutant soybean plantsgrown in a green house. As shown in Table 4, protein is increased (up to9.5%), and oil, fiber and carbohydrates are decreased in QQS-expressingmutant soybean plants grown in a field.

TABLE 2 Growth Chamber Data for R2 Seeds (Year 2010) Mean MoistureChange (% WT) QQS Leaf (%) Protein* Oil* Fiber* P + O* Protein Oil FiberP + O Transcripts⁺ Starch{circumflex over ( )} QQS- 7.83 38.47 18.494.88 56.96 9.93 −5.25 −4.72 4.49 81.7844 4.6401 Williams 82 #1 QQS- 6.5739.33 18.18 4.81 57.51 12.37 −6.81 −6.06 5.51 5.11354 5.2615 Williams 82#2 QQS- 5.93 40.90 17.57 4.78 58.47 16.85 −9.96 −6.72 7.26 2.7319813.638 Williams 82 #3 QQS- 6.93 37.90 18.73 4.81 56.62 8.28 −4.01 −6.063.88 45.3269 14.94 Williams 82 #4 QQS- 5.97 37.56 18.73 4.88 56.28 7.30−4.01 −4.73 3.25 4.57207 2.7128 Williams 82 #5 QQS- 6.40 39.54 17.984.74 57.51 12.96 −7.86 −7.39 5.51 2.62104 5.7704 Williams 82 #6 QQS-7.50 38.89 18.32 4.78 57.20 11.11 −6.11 −6.73 4.94 2.87029 11.014Williams 82 #7 QQS- 10.97 40.01 19.07 4.57 59.08 14.32 −2.27 −10.72 8.382.19775 5.6369 Williams 82 #8 Williams 82 6.73 35.00 19.51 5.12 54.510.00 0.00 0.00 0.00 0 15.561 *data shown for mean protein, oil, fiber,and P + O (protein and oil) are based on % of dry weight ⁺data shown forQQS transcripts are based on QQS/18S rRNA {circumflex over ( )}datashown for leaf starch are based on mg/g fresh weight

TABLE 3 Green House Data for R2 Seeds (Year 2011) Mean Moisture Change(% WT) QQS Leaf (%) Protein* Oil* Fiber* CHO* P + O* Protein Oil FiberCHO P + O Transcripts⁺ Starch{circumflex over ( )} QQS- 7.30 40.57 20.354.54 19.83 60.92 10.39 −2.35 −7.04 −13.06 5.78 0.745933 Williams 82 #10Plant 4 QQS- 7.20 39.25 19.63 4.75 21.67 58.88 6.80 −5.84 −2.78 −5.002.23 0.745933 Williams 82 #10 Plant 8 QQS- 7.70 41.72 18.24 4.65 20.7059.95 13.51 −12.51 −4.92 −9.26 4.09 81.78443 4.64013 Williams 82 #1 QQS-7.10 39.49 20.04 4.68 21.53 59.08 6.24 −3.85 −4.20 −5.61 2.59 81.784434.64013 Williams 82 #1 Plant 5 QQS- 7.47 39.74 18.34 4.82 22.40 58.088.12 −12.00 −1.36 −1.81 0.84 45.32694 14.9403 Williams 82 #4 QQS- 10.9039.49 19.00 4.89 21.91 58.49 7.46 −8.84 0.06 −3.94 1.56 4.572071 2.71278Williams 82 #5 QQS- 9.17 38.94 18.93 4.82 22.61 57.87 5.95 −9.17 −1.36−0.90 0.48 2.621037 5.77036 Williams 82 #2 Plant 1 QQS- 7.70 38.08 20.044.819 22.363 58.12 3.6 −3.9 −1.4 −2.0 0.9 2.870293 11.014 Williams 82 #3Williams 7.60 40.01 19.244 4.681 21.359 59.26 8.9 −7.7 −4.2 −6.4 2.92.197754 5.63693 82 #5 Williams 9.43 36.75 20.84 4.89 22.82 57.59 0.000.00 0.00 0.00 0.00 0 15.5605 82 *data shown for mean protein, oil,fiber, CHO (carbohydrates), and P + O (protein and oil) are based on %of dry weight ⁺data shown for QQS transcripts are based on QQS/18S rRNA{circumflex over ( )}data shown for leaf starch are based on mg/g freshweight

TABLE 4 Field Data for R2/R3 Seeds (Year 2011) Mean Moisture Change (%WT) QQS Leaf (%) Protein* Oil* Fiber* CHO* P + O* Protein Oil Fiber CHOP + O Transcripts⁺ Starch{circumflex over ( )} QQS- 8.5 35.99 17.91 5.1524.87 53.90 3.33 −0.57 −1.95 −3.70 2.00 81.78443 4.64013 Williams 82 #1QQS- 9.7 36.98 17.19 5.08 24.66 54.17 6.17 −4.55 −3.25 −4.49 2.522.621037 5.77036 Williams 82 #6 QQS- 7.0 35.78 17.91 5.12 25.11 53.692.74 −0.57 −2.60 −2.77 1.61 2.870293 11.014 Williams 82 #7 QQS- 7.338.17 16.20 5.05 24.49 54.37 9.50 −10.23 −3.90 −4.89 2.78 0.0316458.73995 Williams 82 #9 Williams 7 34.83 18.01 5.25 25.82 52.84 0.00 0.000.00 0.00 0.00 0 15.5605 82 *data shown for mean protein, oil, fiber CHO(carbohydrates), and P + O (protein and oil) are based on % of dryweight ⁺data shown for QQS transcripts are based on QQS/18S rRNA{circumflex over ( )}data shown for leaf starch are based on mg/g freshweight

While protein content and total protein+oil content increased under allconditions, the increases in protein content and total protein+oilcontent were greater under growth chamber/green house conditionscompared to field conditions. The differences may reflect the fact thatthe soil in the growth chamber and the green house was supplemented withfertilizer (i.e., Miracle Gro Excel 15-5-15), whereas the soil in thefield was not. This study will be repeated with supplementation of soilunder all growth conditions.

All references, including publications, patent applications, andpatents, cited herein are hereby incorporated by reference to the sameextent as if each reference were individually and specifically indicatedto be incorporated by reference and were set forth in its entiretyherein.

The use of the terms “a,” “an,” “the,” and similar referents in thecontext of describing the invention (especially in the context of thefollowing claims) are to be construed to cover both the singular and theplural, unless otherwise indicated herein or clearly contradicted bycontext. Recitation of ranges of values herein are merely intended toserve as a shorthand method of referring individually to each separatevalue falling within the range, unless otherwise indicated herein, andeach separate value is incorporated into the specification as if it wereindividually recited herein. All methods described herein can beperformed in any suitable order unless otherwise indicated herein orotherwise clearly contradicted by context. The use of any and allexamples, or exemplary language (e.g., “such as”) provided herein, isintended merely to illuminate better the invention and does not pose alimitation on the scope of the invention unless otherwise claimed. Nolanguage in the specification should be construed as indicating anynon-claimed element as essential to the practice of the invention.

Preferred embodiments of this invention are described herein, includingthe best mode known to the inventors for carrying out the invention. Itshould be understood that the illustrated embodiments are exemplaryonly, and should not be taken as limiting the scope of the invention.

1.-11. (canceled)
 12. A transgenic plant, or part thereof, whichcomprises and expresses a QQS selected from the group consisting of (i)QQS having the amino acid sequence of SEQ ID NO:2 and (ii) QQS encodedby the nucleotide sequence of SEQ ID NO:1 as a transgene and in which atleast a protein is present in an amount that differs from the amountthat is present in a corresponding WT plant, or part thereof, which doesnot express the QQS.
 13. The plant, or part thereof, of claim 12,wherein the plant is soybean, rice, or corn.
 14. A tissue culture ofregenerable cells of the transgenic plant of claim
 12. 15.-27.(canceled)
 28. The plant, or part thereof, of claim 13, wherein the QQSis under the control of a non-native promoter.
 29. The plant, or partthereof, of claim 28, wherein the non-native promoter is a constitutivepromoter.
 30. The plant, or part thereof, of claim 29, wherein theconstitutive promoter is a cauliflower mosaic virus 35S promoter. 31.The plant, or part thereof, of claim 28, wherein the non-native promoteris an inducible promoter.
 32. The plant or part thereof, of claim 28,wherein the non-native promoter is a developmentally specific promoter.33. The plant or part thereof, of claim 32, wherein the developmentallyspecific promoter is a seed-specific promoter.
 34. The plant or partthereof, of claim 28, wherein the non-native promoter is a syntheticpromoter.
 35. The plant or part thereof, of claim 34, wherein thesynthetic promoter is a hybrid promoter.
 36. The plant, or part thereof,of claim 13, wherein the amount of protein in the seeds of the plant isincreased by at least 30% as compared to the amount of protein in theseeds of wild-type (WT) soybean.
 37. The plant, or part thereof, ofclaim 13, wherein the amount of protein in the seeds of the plant isincreased by at least 45% as compared to the amount of protein in theseeds of WT soybean.
 38. The plant, or part thereof, of claim 13,wherein the amount of protein in the seeds of the plant is increased byat least 60% as compared to the amount of protein in the seeds of WTsoybean.
 39. A tissue culture of regenerable cells of the transgenicplant of claim 13.